pAAV-cTNT-Ogt plasmid

pAAV-cTNT-Ogt plasmid

PVT88769

pAAV-cTNT-Ogt plasmid Information

Plasmid name: pAAV-cTNT-Ogt(mouse)
Alias: NM_139144.4;1110038P24Rik; 4831420N21Rik; Ogtl
Gene lenght: 3138bp
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: chimeric intron-F
Reverse primer:
Copy number: High
Induction:

pAAV-cTNT-Ogt plasmid Description

pAAV-cTNT-Ogt This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-cTNT-Ogt plasmid Sequence

1 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA 61 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA 121 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT 181 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA 241 GATTTAATTA AGGCCTTAAT TAGGaggaac ccctagtgat ggagttggcc actccctctc 301 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg 361 cccgggcggc ctcagtgagc gagcgagcgc gcagTGTAGT TAATGATTAA CCCGCCATGC 421 TACTTATCTA CCAGGGTAAT GGGGATCCTC TAGAACTATA GCTAGAATTC GCCCTTACGG 481 GCCCCCCCTC GAGGTCGGGA TAAAAGCAGT CTGGGCTTTC ACATGACAGC ATCTGGGGCT 541 GCGGCAGAGG GTCGGGTCCG AAGCGCTGCC TTATCAGCGT CCCCAGCCCT GGGAGGTGAC 601 AGCTGGCTGG CTTGTGTCAG CCCCTCGGGC ACTCACGTAT CTCCGTCCGA CGGGTTTAAA 661 ATAGCAAAAC TCTGAGGCCA CACAATAGCT TGGGCTTATA TGGGCTCCTG TGGGGGAAGG 721 GGGAGCACGG AGGGGGCCGG GGCCGCTGCT GCCAAAATAG CAGCTCACAA GTGTTGCATT 781 CCTCTCTGGG CGCCGGGCAC ATTCCTGCTG GCTCTGCCCG CCCCGGGGTG GGCGCCGGGG 841 GGACCTTAAA GCCTCTGCCC CCCAAGGAGC CCTTCCCAGA CAGCCGCCGG CACCCACCGC 901 TCCGTGGGAC GATCCCCGAA GCTCTAGAGC TTTATTGCGG TAGTTTATCA CAGTTAAATT 961 GCTAACGCAG TCAGTGCTTC TGACACAACA GTCTCGAACT TAAGCTGCAG AAGTTGGTCG 1021 TGAGGCACTG GGCAGGTAAG TATCAAGGTT ACAAGACAGG TTTAAGGAGA CCAATAGAAA 1081 CTGGGCTTGT CGAGACAGAG AAGACTCTTG CGTTTCTGAT AGGCACCTAT TGGTCTTACT 1141 GACATCCACT TTGCCTTTCT CTCCACAGGT GTCCACTCCC AGTTCAATTA CAGCTCTTAA 1201 GGCTAGAGTA CTTAATACGA CTCACTATAG GCTAGCgcca ccATGGCGTC TTCCGTGGGC 1261 AACGTGGCCG ACAGTACAGA ACCAACGAAA CGTATGCTTT CCTTCCAAGG GTTAGCTGAG 1321 TTGGCACATC GAGAATATCA GGCAGGAGAT TTTGAGGCAG CTGAGAGACA CTGCATGCAG 1381 CTCTGGAGAC AAGAGCCTGA CAATACTGGT GTTCTTTTAT TACTTTCATC TATACACTTC 1441 CAGTGTCGAA GGCTGGACAG ATCTGCTCAT TTTAGCACCT TGGCAATTAA ACAGAATCCC 1501 CTTCTAGCAG AAGCCTATTC GAATTTGGGA AATGTGTACA AGGAAAGAGG GCAGTTGCAG 1561 GAAGCAATCG AGCATTATCG ACATGCCTTG CGGCTGAAGC CTGATTTCAT TGATGGTTAT 1621 ATTAACCTGG CAGCAGCCTT GGTAGCAGCA GGTGACATGG AAGGAGCAGT ACAAGCCTAT 1681 GTCTCTGCTC TTCAGTACAA TCCTGATTTG TACTGTGTTC GCAGTGACCT GGGGAACCTG 1741 CTCAAAGCCC TGGGTCGCTT GGAAGAAGCC AAGGCATGTT ATTTGAAAGC AATTGAGACG 1801 CAACCAAACT TTGCAGTAGC CTGGAGTAAT CTCGGCTGTG TTTTCAATGC ACAAGGGGAG 1861 ATTTGGCTGG CTATTCATCA CTTTGAAAAG GCTGTCACCC TTGACCCAAA TTTTCTGGAT 1921 GCTTATATCA ATTTAGGAAA TGTCTTGAAA GAGGCACGCA TTTTTGACAG AGCTGTCGCA 1981 GCTTATCTTC GTGCCTTAAG TTTGAGCCCA AATCATGCGG TGGTGCACGG CAACCTGGCT 2041 TGTGTGTACT ACGAGCAAGG CCTAATAGAC CTGGCCATTG ATACCTACAG GAGAGCTATC 2101 GAACTGCAAC CCCATTTCCC CGATGCTTAC TGCAACCTAG CAAATGCTCT CAAAGAGAAG 2161 GGCAGTGTTG CTGAAGCAGA AGATTGTTAT AACACAGCTC TTCGTCTGTG TCCTACTCAT 2221 GCAGACTCTT TGAATAACCT TGCCAACATC AAACGGGAAC AGGGCAACAT TGAAGAGGCA 2281 GTTCGCCTGT ATCGCAAAGC ATTAGAAGTC TTCCCAGAGT TTGCTGCTGC ACATTCCAAT 2341 TTAGCAAGTG TACTGCAACA GCAGGGCAAG CTGCAGGAAG CACTGATGCA CTATAAAGAA 2401 GCCATACGAA TTAGTCCTAC ATTTGCTGAT GCTTATTCCA ATATGGGAAA CACTCTAAAG 2461 GAGATGCAGG ATGTGCAGGG CGCTTTGCAG TGTTATACTC GTGCCATCCA GATTAATCCT 2521 GCCTTTGCTG ATGCACACAG CAATCTGGCC TCCATTCACA AGGATTCAGG GAATATCCCA 2581 GAAGCAATAG CTTCTTACCG CACAGCTCTG AAACTTAAGC CTGACTTTCC TGATGCTTAT 2641 TGTAACTTGG CTCATTGCCT ACAGATTGTC TGTGATTGGA CAGACTATGA TGAGCGGATG 2701 AAGAAATTGG TTAGTATTGT AGCTGAGCAG CTAGAGAAGA ATAGACTGCC TTCTGTCCAT 2761 CCTCACCATA GCATGCTGTA CCCTCTTTCC CATGGCTTCA GGAAGGCTAT TGCAGAGAGG 2821 CATGGGAATC TCTGCTTGGA TAAGATTAAT GTCCTTCATA AACCACCATA TGAACATCCA 2881 AAAGACTTGA AGCTCAGTGA TGGCCGATTG CGTGTAGGCT ATGTGAGTTC TGACTTCGGG 2941 AATCACCCTA CTTCACACCT TATGCAGTCT ATTCCAGGCA TGCATAATCC TGATAAGTTT 3001 GAGGTATTCT GCTATGCCTT GAGCCCGGAT GATGGTACAA ACTTTCGAGT GAAGGTGATG 3061 GCGGAAGCCA ATCATTTCAT TGATCTTTCT CAGATTCCTT GTAATGGAAA AGCAGCCGAC 3121 CGCATCCACC AAGATGGAAT TCACATCCTT GTGAATATGA ATGGGTATAC CAAGGGTGCT 3181 CGGAATGAGC TCTTTGCTCT TAGGCCAGCT CCTATTCAGG CCATGTGGCT GGGCTACCCT 3241 GGGACTAGTG GTGCACTGTT CATGGATTAC ATCATCACTG ATCAGGAAAC TTCCCCAGCT 3301 GAAGTTGCAG AGCAGTATTC TGAGAAACTG GCTTATATGC CCCATACTTT CTTTATTGGT 3361 GATCATGCTA ATATGTTCCC TCACCTGAAG AAAAAAGCAG TCATCGATTT TAAATCCAAT 3421 GGGCACATTT ATGATAATCG GATAGTTCTG AATGGCATCG ATCTCAAAGC ATTTCTCGAT 3481 AGCCTACCCG ATGTGAAGAT TGTCAAGATG AAATGTCCTG ATGGAGGTGA CAATCCAGAC 3541 AGCAGTAACA CAGCTCTTAA TATGCCCGTT ATTCCCATGA ATACGATTGC AGAAGCAGTA 3601 ATTGAAATGA TTAACAGAGG GCAGATTCAG ATAACAATTA ACGGATTCAG TATTAGCAAT 3661 GGACTGGCGA CTACACAGAT TAATAATAAG GCTGCAACCG GAGAGGAAGT TCCCCGTACC 3721 ATTATTGTAA CCACCCGTTC CCAGTATGGG CTACCAGAAG ATGCCATTGT GTACTGTAAC 3781 TTTAATCAGT TATATAAAAT TGACCCATCT ACCCTGCAGA TGTGGGCAAA TATTCTGAAA 3841 CGTGTGCCTA ACAGCGTGCT TTGGCTGTTG CGTTTTCCAG CAGTAGGAGA ACCCAATATT 3901 CAACAATATG CACAAAATAT GGGCCTTCCC CAGAACCGTA TCATTTTCTC ACCTGTGGCT 3961 CCTAAAGAGG AGCATGTCAG GAGAGGTCAG CTGGCTGATG TCTGCCTGGA TACTCCTTTG 4021 TGTAATGGAC ACACCACAGG GATGGATGTT CTCTGGGCAG GAACACCCAT GGTGACTATG 4081 CCAGGAGAGA CTCTTGCCTC TCGAGTTGCA GCTTCTCAGC TTACTTGTCT AGGATGTCTC 4141 GAGCTCATTG CTAAAAGCAG ACAGGAATAT GAAGACATAG CTGTGAAACT GGGAACCGAT 4201 CTAGAATACC TGAAGAAAAT TCGTGGCAAA GTCTGGAAAC AGAGAATATC TAGCCCTCTG 4261 TTCAACACCA AACAATACAC AATGGAATTA GAGCGACTTT ATCTGCAGAT GTGGGAGCAT 4321 TATGCAGCTG GCAACAAACC TGACCACATG ATTAAGCCTG TTGAAGTCAC CGAGTCAGCC 4381 tGAGTCGACC CGGGCGGCCT CGAGGACGGG GTGAACTACG CCTGAATCAA GCTTATCGAT 4441 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 4501 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 4561 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 4621 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 4681 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 4741 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 4801 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 4861 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 4921 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 4981 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 5041 CGATGAGGAT CCGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT GAAGCCCCTT 5101 GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT 5161 TTTTTGTGTC TCTCACTCGG AAGCAATTCG TTGATCTGAA TTTCGACCAC CCATAATACC 5221 CATTACCCTG GTAGATAAGT AGCATGGCGG GTTAATCATT AACTACAagg aacccctagt 5281 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa 5341 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagCCT 5401 TAATTAACCT AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT 5461 TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA 5521 GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGGACGCGCC 5581 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT 5641 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC 5701 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT 5761 ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC 5821 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT 5881 GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT 5941 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 6001 TTTTAACAAA ATATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 6061 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 6121 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 6181 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 6241 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 6301 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 6361 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 6421 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 6481 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 6541 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 6601 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 6661 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 6721 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 6781 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 6841 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 6901 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 6961 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 7021 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 7081 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 7141 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 7201 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 7261 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 7321 CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 7381 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 7441 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 7501 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 7561 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 7621 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 7681 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 7741 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 7801 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 7861 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 7921 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.