pCMV-SEC31A-3×HA-Neo plasmid

pCMV-SEC31A-3×HA-Neo plasmid

PVT88504

pCMV-SEC31A-3×HA-Neo plasmid Information

Plasmid name: pCMV-SEC31A(human)-3×HA-Neo
Alias: NM_001077207.4;ABP125; ABP130; HPBKS; HSPC275; HSPC334;NEDSOSB; SEC31L1;
Gene lenght: 3660bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SEC31A-3×HA-Neo plasmid Description

pCMV-SEC31A-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SEC31A-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgaa gttaaaggaa 721 gtagatcgta cagccatgca ggcatggagc cctgcccaga atcaccccat ttacctagca 781 acaggaacat ctgctcagca attggatgca acatttagta cgaatgcttc ccttgagata 841 tttgaattag acctctctga tccatccttg gatatgaaat cttgtgccac attctcctct 901 tctcacaggt accacaagtt gatttggggg ccttataaaa tggattccaa aggagatgtc 961 tctggagttc tgattgcagg tggtgaaaat ggaaatatta ttctctatga tccttctaaa 1021 attatagctg gagacaagga agttgtgatt gcccagaatg acaagcatac tggcccagtg 1081 agagccttgg atgtgaacat tttccagact aatctggtag cttctggtgc taatgaatct 1141 gaaatctaca tatgggatct aaataatttt gcaaccccaa tgacaccagg agccaaaaca 1201 cagccgccag aagatatcag ctgcattgca tggaacagac aagttcagca tattttagca 1261 tcagccagtc ccagtggccg ggccactgta tgggatctta gaaaaaatga gccaatcatc 1321 aaagtcagtg accatagtaa cagaatgcat tgttctgggt tggcatggca tcctgatgtt 1381 gctactcaga tggtccttgc ctccgaggat gaccggttac cagtgatcca gatgtgggat 1441 cttcgatttg cttcctctcc acttcgtgtc ctggaaaacc atgccagggg gattttggca 1501 attgcttgga gcatggcaga tcctgaattg ttactgagct gtggaaaaga tgctaagatt 1561 ctctgctcca atccaaacac aggagaggtg ttatatgaac ttcccaccaa cacacagtgg 1621 tgcttcgata ttcagtggtg tccccgaaat cctgctgtct tatcagctgc ttcgtttgat 1681 gggcgtatca gtgtttattc tatcatggga ggtagcacag atggtttaag acagaaacaa 1741 gttgacaagc tttcatcatc ttttgggaat cttgatccct ttggcacagg acagcccctt 1801 cctccgttac aaattccaca gcagactgct cagcatagta tagtgctgcc tctgaagaag 1861 ccgcccaagt ggattcgaag gcctgttggt gcttcttttt catttggagg caaactggtt 1921 acgtttgaga atgtcagaat gccttctcat cagggagctg agcagcagca gcagcagcac 1981 catgtgttca ttagtcaggt tgtaacagaa aaggagttcc tcagccgatc agaccaactt 2041 cagcaggctg tgcagtcaca aggatttatc aattattgcc aaaaaaaaat tgatgcttct 2101 cagactgaat ttgagaaaaa tgtgtggtcc tttttgaagg taaactttga ggatgattct 2161 cgtggaaaat accttgaact tctaggatac agaaaagaag atctaggaaa gaagattgct 2221 ttggccttga acaaagtgga tggagccaat gtggctctta aagactctga ccaagtagca 2281 cagagtgatg gggaggagag ccctgctgct gaagagcagc tcttgggaga gcacattaaa 2341 gaggaaaaag aagaatctga atttctaccc tcatctggag gaacatttaa tatctctgtc 2401 agtggggaca ttgatggttt aattactcag gctttgctga cgggcaattt tgagagtgct 2461 gttgaccttt gtttacatga taaccgcatg gccgatgcca ttatattggc catagcaggt 2521 ggacaagaac tcttggctcg aacccagaaa aaatacttcg caaaatccca aagcaaaatt 2581 accaggctca tcactgcagt ggtgatgaag aactggaaag agattgttga gtcttgtgat 2641 cttaaaaatt ggagagaggc tttagctgca gtattgactt atgcaaagcc ggatgaattt 2701 tcagcccttt gtgatctttt gggaaccagg cttgaaaatg aaggagatag cctcctgcag 2761 actcaagcat gtctctgcta tatttgtgca gggaatgtag agaaattagt tgcatgttgg 2821 actaaagctc aagatggaag ccaccctttg tcacttcagg atctgattga gaaagttgtc 2881 atcctgcgaa aagctgtgca actcactcaa gccatggaca ctagtactgt aggagttctc 2941 ttggctgcga agatgagtca gtatgccaat ttgttggcag ctcagggcag tattgctgca 3001 gccttggctt ttcttcctga caacaccaac cagccaaata tcatgcagct tcgtgacaga 3061 ctttgtagag cacaaggaga gcctgtagca ggacatgaat cacctaaaat tccgtacgag 3121 aaacagcagc tccccaaggg caggcctgga ccagttgctg gccaccacca gatgccaaga 3181 gttcaaactc aacaatatta tccccatgga gaaaatcctc cacctccggg tttcataatg 3241 catggaaatg ttaatccaaa tgctgctggt cagcttccca catctccagg tcatatgcac 3301 acccaggtac caccttatcc acagccacag ccttatcaac cagcccagcc gtatcccttc 3361 ggaacagggg ggtcagcaat gtatcgacct cagcagcctg ttgctcctcc tacttcaaac 3421 gcttacccta acacccctta catatcttct gcttcttcct atactgggca gtctcagctg 3481 tacgcagcac agcaccaggc ctcttcacct acctccagcc ctgctacttc tttccctcct 3541 cccccttcct ctggagcatc cttccagcat ggcggaccag gagctccacc atcatcttca 3601 gcttatgcac tgcctcctgg aacaacaggt acactgcctg ctgccagtga gctgcctgcg 3661 tcccaaagaa caggtcctca gaatggttgg aatgaccctc cagctttgaa cagagtaccc 3721 aaaaagaaga agatgcctga aaacttcatg cctcctgttc ccatcacatc accaatcatg 3781 aacccgttgg gtgaccccca gtcacaaatg ctgcagcaac agccttcagc tccagtacca 3841 ctgtcaagcc agtcttcatt cccacagcca catcttccag gtggccagcc cttccatggc 3901 gtacagcaac ctcttggtca aacaggcatg ccaccatctt tttcaaagcc caatattgaa 3961 ggtgccccag gggctcctat tggaaatacc ttccagcatg tgcagtcttt gccaacaaaa 4021 aaaattacca agaaacctat tccagatgag cacctcattc taaagaccac atttgaggat 4081 cttattcagc gctgcctttc ttcagcaaca gaccctcaaa ccaagaggaa gctagatgat 4141 gccagcaaac gtttggagtt tctgtatgat aaacttaggg aacagacact ttcaccaaca 4201 atcaccagtg gtttacacaa cattgcaagg agcattgaaa ctcgaaacta ctcagaagga 4261 ttgaccatgc atacccacat agttagcacc agcaacttca gtgagacctc tgctttcatg 4321 ccagttctca aagttgttct cacccaggcc aataagctgg gtgtcCTCGA GTACCCATAC 4381 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 4441 GATGTCCCAG ACTACGCATA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA 4501 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 4561 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 4621 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 4681 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 4741 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 4801 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 4861 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 4921 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 4981 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 5041 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG 5101 GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC 5161 GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG 5221 TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC 5281 CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 5341 CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG 5401 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 5461 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 5521 CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 5581 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 5641 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 5701 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA 5761 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT 5821 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 5881 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 5941 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC 6001 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 6061 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 6121 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 6181 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG 6241 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 6301 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 6361 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 6421 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 6481 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 6541 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 6601 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 6661 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 6721 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 6781 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 6841 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG 6901 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT 6961 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC 7021 GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 7081 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 7141 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 7201 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 7261 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 7321 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 7381 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 7441 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 7501 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 7561 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 7621 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 7681 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 7741 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 7801 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 7861 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 7921 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC 7981 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 8041 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 8101 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 8161 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 8221 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 8281 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 8341 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 8401 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 8461 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 8521 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 8581 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 8641 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 8701 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 8761 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 8821 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 8881 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 8941 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 9001 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 9061 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 9121 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 9181 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 9241 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 9301 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 9361 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 9421 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 9481 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC 9541 CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA 9601 TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC 9661 AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.