pLV3-CMV-CAMK1D-3×FLAG-CopGFP-

pLV3-CMV-CAMK1D-3×FLAG-CopGFP-Puro plasmid

PVT88381

pLV3-CMV-CAMK1D-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CAMK1D(human)-3×FLAG-CopGFP-Puro
Alias: NM_020397.4;CaM-K1; CaMKID; CKLiK
Gene lenght: 1071bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CAMK1D-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CAMK1D-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CAMK1D-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccATGG CCCGGGAGAA CGGCGAGAGC 2341 AGCTCCTCCT GGAAAAAGCA AGCTGAAGAC ATCAAGAAGA TCTTCGAGTT CAAAGAGACC 2401 CTCGGAACCG GGGCCTTTTC CGAAGTGGTT TTAGCTGAAG AGAAGGCAAC TGGCAAGCTC 2461 TTTGCTGTGA AGTGTATCCC TAAGAAGGCG CTGAAGGGCA AGGAAAGCAG CATAGAGAAT 2521 GAGATAGCCG TCCTGAGAAA GATTAAGCAT GAAAATATTG TTGCCCTGGA AGACATTTAT 2581 GAAAGCCCAA ATCACCTGTA CTTGGTCATG CAGCTGGTGT CCGGTGGAGA GCTGTTTGAC 2641 CGGATAGTGG AGAAGGGGTT TTATACAGAG AAGGATGCCA GCACTCTGAT CCGCCAAGTC 2701 TTGGACGCCG TGTACTATCT CCACAGAATG GGCATCGTCC ACAGAGACCT CAAGCCCGAA 2761 AATCTCTTGT ACTACAGTCA AGATGAGGAG TCCAAAATAA TGATCAGTGA CTTTGGATTG 2821 TCAAAAATGG AGGGCAAAGG AGATGTGATG TCCACTGCCT GTGGAACTCC AGGCTATGTC 2881 GCTCCTGAAG TCCTCGCCCA GAAACCTTAC AGCAAAGCCG TTGACTGCTG GTCCATCGGA 2941 GTGATTGCCT ACATCTTGCT CTGCGGCTAC CCTCCTTTTT ATGATGAAAA TGACTCCAAG 3001 CTCTTTGAGC AGATCCTCAA GGCGGAATAT GAGTTTGACT CTCCCTACTG GGATGACATC 3061 TCCGACTCTG CAAAAGACTT CATTCGGAAC CTGATGGAGA AGGACCCGAA TAAAAGATAC 3121 ACGTGTGAGC AGGCAGCTCG GCACCCATGG ATCGCTGGTG ACACAGCCCT CAACAAAAAC 3181 ATCCACGAGT CCGTCAGCGC CCAGATCCGG AAAAACTTTG CCAAGAGCAA ATGGAGACAA 3241 GCATTTAATG CCACGGCCGT CGTCAGACAT ATGAGAAAAC TACACCTCGG CAGCAGCCTG 3301 GACAGTTCAA ATGCAAGTGT TTCGAGCAGC CTCAGTTTGG CCAGCCAAAA AGACTGTGCG 3361 TATGTAGCAA AACCAGAATC CCTCAGCgga tccGACTACA AAGACCATGA CGGTGATTAT 3421 AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGt agcggccgcG aaggatctgc 3481 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg 3541 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa 3601 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt 3661 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag 3721 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca 3781 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc 3841 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc 3901 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc 3961 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta 4021 gacgccacca tggagagcga cgagagcggc ctgcccgcca tggagatcga gtgccgcatc 4081 accggcaccc tgaacggcgt ggagttcgag ctggtgggcg gcggagaggg cacccccaag 4141 cagggccgca tgaccaacaa gatgaagagc accaaaggcg ccctgacctt cagcccctac 4201 ctgctgagcc acgtgatggg ctacggcttc taccacttcg gcacctaccc cagcggctac 4261 gagaacccct tcctgcacgc catcaacaac ggcggctaca ccaacacccg catcgagaag 4321 tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc gctacgaggc cggccgcgtg 4381 atcggcgact tcaaggtggt gggcaccggc ttccccgagg acagcgtgat cttcaccgac 4441 aagatcatcc gcagcaacgc caccgtggag cacctgcacc ccatgggcga taacgtgctg 4501 gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg gctactacag cttcgtggtg 4561 gacagccaca tgcacttcaa gagcgccatc caccccagca tcctgcagaa cgggggcccc 4621 atgttcgcct tccgccgcgt ggaggagctg cacagcaaca ccgagctggg catcgtggag 4681 taccagcacg ccttcaagac ccccatcgcc ttcgccagat cccgcgctca gtcgtccaat 4741 tctgccgtgg acggcaccgc cggacccggc tccaccggat ctcgcgaggg cagaggaagt 4801 cttctaacat gcggtgacgt ggaggagaat cccggcccta tgaccgagta caagcccacg 4861 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 4921 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 4981 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 5041 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 5101 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 5161 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 5221 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 5281 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 5341 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 5401 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa atcaacctct ggattacaaa 5461 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 5521 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 5581 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 5641 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 5701 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 5761 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 5821 gtgttgtcgg ggaaATCATC GTCCTTTCCT tggctgctcg cctgtgttgc cacctggatt 5881 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 5941 cgcggcctgc tgccggctct gcggcctctt ccgcgtctTc gccttcgccc tcagacgagt 6001 cggatctccc tttggGccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc 6061 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc 6121 ccaacgaaGa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg 6181 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 6241 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 6301 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat 6361 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc 6421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt 6481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct 6541 ctagctaTCC CGCCCCTAAC TCCGCCCAtc ccgcccctaa ctccgcccag ttccgcccat 6601 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc 6661 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac 6721 caaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 6781 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 6841 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6901 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6961 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7141 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7321 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7561 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7741 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7801 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7861 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7921 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7981 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8041 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8101 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8161 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8221 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8281 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8341 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8401 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8461 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8521 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8581 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8641 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8701 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 8761 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 8821 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8881 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8941 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 9001 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 9061 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 9121 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 9181 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 9241 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 9301 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 9361 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.