pCMV-Card9 plasmid

pCMV-Card9 plasmid

PVT88372

pCMV-Card9 plasmid Information

Plasmid name: pCMV-Card9(mouse)
Alias: NM_001037747.3;Gm782
Gene lenght: 1608bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Card9 plasmid Description

pCMV-Card9 is a plasmid suitable for protein expression in mammalian cells.

pCMV-Card9 plasmid Sequence

1 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag 61 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 121 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 181 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 241 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 301 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 361 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg 421 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc 481 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa 541 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc 601 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt 661 tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct tacatgtggt 721 accgagctcg gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc 781 cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat 841 attgaccaaa tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat 901 atactttttt gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata 961 atgatacaat gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg 1021 gttaaggcaa tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg 1081 taagaggttt catattgcta atagcagcta caatccagct accattctgc ttttatttta 1141 tggttgggat aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc 1201 atacctctta tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat 1261 cactttggca aagcacgtga gatctgaatt cGCCACCATG TCAGACTATG AGAATGACGA 1321 CGAGTGCTGG AGCACCCTGG AGAGCTTCCG GGTGAAGCTC ATCTCTGTCA TTGACCCCTC 1381 CCGGATCACA CCCTATCTAC GCCAGTGCAA AGTCCTGAAC CCCGATGATG AGGAGCAGGT 1441 GCTCAGTGAC CCCAACCTGG TCATCCGCAA GCGGAAAGTG GGTGTGCTCC TGGACATCCT 1501 GCAGCGGACA GGCCACAAGG GCTACGTGGC TTTCCTCGAG AGCCTGGAGC TCTACTACCC 1561 TCAGTTATAC CGGAAAGTCA CTGGCAAGGA GCCAGCACGC GTCTTCTCCA TGATCATTGA 1621 TGCATCAGGG GAGTCTGGCC TGACGCAGCT GCTGATGACA GAGGTCATGA AGCTGCAGAA 1681 GAAGGTTCAG GACCTGACGG CCCTTCTGAG CTCCAAGGAC GACTTCATCA AGGAGCTGAG 1741 GGTAAAGGAC AGCCTACTGC GCAAGCACCA GGAGCGGGTG CAGCGGCTCA AGGAGGAGTG 1801 TGAGCTGAGC AGTGCGGAGC TGAAGCGCTG CAAGGACGAG AACTATGAGC TGGCCATGTG 1861 CCTGGCACAT CTGAGTGAAG AGAAGGGCGC AGCACTCATG CGGAACCGTG ACCTGCAGCT 1921 TGAGGTGGAC CGGCTCAGGC ACAGCCTCAT GAAGGCCGAG GATGACTGCA AGGTGGAGCG 1981 CAAACACACA CTGAAGCTCA GGCACGCCAT GGAGCAGCGG CCTAGTCAGG AGCTGCTGTG 2041 GGAACTACAG CAGGAAAAGG ACTTGCTGCA GGCCCGGGTG CAGGAGCTGC AGGTCTCTGT 2101 GCAGGAGGGT AAGCTAGACA GGAATAGTCC ATACATTCAA GTGCTGGAGG AGGACTGGCG 2161 TCAAGCACTG CAGGAACACC AGAAGCAGGT CAGCACCATC TTCTCCCTAC GGAAGGACCT 2221 CCGCCAGGCT GAGACCCTCC GGGCCCGGTG CACGGAAGAA AAGGAGATGT TCGAGCTGCA 2281 GTGCCTGGCC TTGCGCAAGG ATGCCAAGAT GTACAAGGAC CGTATCGAGG CTATCCTGCT 2341 GCAGATGGAG GAGGTCTCCA TTGAGAGGGA CCAGGCTATG GCCTCCAGGG AAGAGCTGCA 2401 TGCACAGTGT ACCCAAAGCT TTCAGGACAA AGATAAGCTT CGAAAGCTGG TTCGAGAGCT 2461 GGGTGAGAAG GCAGATGAGC TGCAGCTACA GCTGTTCCAG ACGGAGAGCC GATTACTGGC 2521 CGCCGAGGGC AGACTCAAGC AGCAGCAATT GGACATGCTC ATCCTGAGCT CTGACTTGGA 2581 AGACAGTTCA CCCAGGAACT CCCAGGAGCT CTCACTGCCT CAGGATCTGG AGGAGGATGC 2641 CCAGCTCTCA GACAAAGGTG TACTGGCAGA CAGGGAGAGC CCAGAGCAGC CCTTTATGGC 2701 TCTGAACAAG GAGCATCTTT CACTGACCCA TGGCATGGGG CCCAGCAGCA GCGAGCCCCC 2761 GGAGAAGGAG CGGCGGCGCC TCAAGGAGAG CTTCGAGAAC TACCGCAGGA AGCGGGCGCT 2821 CCGCAAGATG CAGAACAGCT GGCGGCAGGG AGAAGGGGAT CGCGGGAATA CGACAGGCAG 2881 CGACAACACC GACACCGAGG GCTCCgGCGG CGGAGGGAGT ATGCGTGACC ACATGGTCCT 2941 TCATGAGTAT GTAAATGCTG CCGGTATCAC Ctaagaattc accccaccag tgcaggctgc 3001 ctatcagaaa gtggtggctg gtgtggctaa tgccctggcc cacaagtatc actaagctcg 3061 ctttcttgct gtccaatttc tattaaaggt tcctttgttc cctaagtcca actactaaac 3121 tgggggatat tatgaagggc cttgagcatc tggattctgc ctaataaaaa acatttattt 3181 tcattgcaat gatgtattta aattatttct gaatatttta ctaaaaaggg aatgtgggag 3241 gtcagtgcat ttaaaacata aagaaatgaa gagctagttc aaaccttggg aaaatacact 3301 atatcttaaa ctccatgaaa gaaggtgagg ctgcaaacag ctaatgcaca ttggcaacag 3361 cccctgatgc ctatgcctta ttcatccctc agaaaaggat tcaagtagag gcttgatttg 3421 gaggttaaag ttttgctatg ctgtatttta cattacttat tgttttagct gtcctcatga 3481 atgtcttttc actacccatt tgcttatcct gcatctctca gccttgactc cactcagttc 3541 tcttgcttag agataccacc tttcccctga agtgttcctt ccatgtttta cggcgagatg 3601 gtttctcctc gcctggccac tcagccttag ttgtctctgt tgtcttatag aggtctactt 3661 gaagaaggaa aaacaggggg catggtttga ctgtcctgtg agcccttctt ccctgcctcc 3721 cccactcaca gtgacccgga atccctcgac atggcagtct agcactagtg cggccgcaga 3781 tctgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 3841 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 3901 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 3961 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 4021 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 4081 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 4141 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 4201 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 4261 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 4321 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 4381 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 4441 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 4501 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 4561 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 4621 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 4681 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 4741 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 4801 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 4861 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 4921 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 4981 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 5041 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 5101 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 5161 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 5221 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 5281 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 5341 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 5401 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 5461 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 5521 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 5581 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 5641 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 5701 gtgccacctg acgtggatcc cctgaggggg cccccatggg ctagaggatc cggcctcggc 5761 ctctgcataa ataaaaaaaa ttagtcagcc atgagcttgg cccattgcat acgttgtatc 5821 catatcataa tatgtacatt tat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.