pLV3-CMV-MDM4-3×FLAG-CopGFP-Pu

pLV3-CMV-MDM4-3×FLAG-CopGFP-Puro plasmid

PVT88361

pLV3-CMV-MDM4-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MDM4(human)-3×FLAG-CopGFP-Puro
Alias: NM_002393.5;BMFS6; HDMX; MDMX; MRP1;
Gene lenght: 1470bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MDM4-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-MDM4-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-MDM4-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga catcattttc cacctctgct 2341 cagtgttcaa catctgacag tgcttgcagg atctctcctg gacaaatcaa tcaggtacga 2401 ccaaaactgc cgcttttgaa gattttgcat gcagcaggtg cgcaaggtga aatgttcact 2461 gttaaagagg tcatgcacta tttaggtcag tacataatgg tgaagcaact ttatgatcag 2521 caggagcagc atatggtata ttgtggtgga gatcttttgg gagaactact gggacgtcag 2581 agcttctccg tgaaagaccc aagccctctc tatgatatgc taagaaagaa tcttgtcact 2641 ttagccactg ctactacaga tgctgctcag actctcgctc tcgcacagga tcacagtatg 2701 gatattccaa gtcaagacca actgaagcaa agtgcagagg aaagttccac ttccagaaaa 2761 agaactacag aagacgatat ccccacactg cctacctcag agcataaatg catacattct 2821 agagaagatg aagacttaat tgaaaattta gcccaagatg aaacatctag gctggacctt 2881 ggatttgagg agtgggatgt agctggcctg ccttggtggt ttttaggaaa cttgagaagc 2941 aactatacac ctagaagtaa tggctcaact gatttacaga caaatcagga tgtgggtact 3001 gccattgttt cagatactac agatgacttg tggtttttga atgagtcagt atcagagcag 3061 ttaggtgttg gaataaaagt tgaagctgct gatactgaac aaacaagtga agaagtaggg 3121 aaagtaagtg acaaaaaggt gattgaagtg ggaaaaaatg atgacctgga ggactctaag 3181 tccttaagtg atgataccga tgtagaggtt acctctgagg atgagtggca gtgtactgaa 3241 tgcaagaaat ttaactctcc aagcaagagg tactgttttc gttgttgggc cttgaggaag 3301 gattggtatt cagattgttc aaagttaacc cattctctct ccacgtctga tatcactgcc 3361 atacctgaaa aggaaaatga aggaaatgat gtccctgatt gtcgaagaac catttcggct 3421 cctgtcgtta gacctaaaga tgcgtatata aagaaagaaa actccaaact ttttgatccc 3481 tgcaactcag tggaattctt ggatttggct cacagttctg aaagccaaga gaccatctca 3541 agcatgggag aacagttaga taacctttct gaacagagaa cagatacaga aaacatggag 3601 gattgccaga atctcttgaa gccatgtagc ttatgtgaga aaagaccacg agacgggaac 3661 attattcatg gaaggacggg ccatcttgtc acttgttttc actgtgccag aagactaaag 3721 aaggctgggg cttcatgccc tatttgcaag aaagagattc agctggttat taaggttttt 3781 atagcaggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3841 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3901 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3961 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 4021 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 4081 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 4141 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 4201 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 4261 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 4321 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4381 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4441 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4501 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4561 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4621 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4681 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4741 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4801 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4861 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4921 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4981 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 5041 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 5101 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 5161 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 5221 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 5281 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 5341 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5401 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5461 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5521 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5581 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5641 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5701 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5761 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5821 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5881 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5941 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6001 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6061 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6121 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6181 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 6241 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6301 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6361 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 6421 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6481 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6541 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6601 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6661 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6721 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6781 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6841 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6901 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6961 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 7021 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 7081 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 7141 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 7201 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 7261 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7321 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7381 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7441 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7501 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7561 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7621 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7681 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7741 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7801 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7861 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7921 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7981 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 8041 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 8101 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8161 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 8221 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8281 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8341 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8401 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8461 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8521 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8581 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8641 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8701 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8761 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8821 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8881 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8941 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 9001 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 9061 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 9121 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 9181 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9241 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9301 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9361 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9421 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9481 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9541 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9601 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9661 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9721 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.