pLV3-CMV-Itgbl1-3×FLAG-CopGFP-

pLV3-CMV-Itgbl1-3×FLAG-CopGFP-Puro plasmid

PVT87921

pLV3-CMV-Itgbl1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Itgbl1(rat)-3×FLAG-CopGFP-Puro
Alias: NM_001017505.1
Gene lenght: 1485bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Itgbl1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Itgbl1-3×FLAG-CopGFP-Puro This is a plasmid suitable for overexpression in mammalian cells. The plasmid contains a CMV strong promoter that can initiate the transcription of the mouse Itgbl1 gene, promoting the C-terminal fusion expression of a 3×FLAG tag. This facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid also carries a Puro resistance gene for selecting host cells that express the target gene.

pLV3-CMV-Itgbl1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgc atcccccagg ctttaagaac 2341 ttcttgttgc tggtgtcctc ccttttcttc attgggctgt cagctgcacc tcaaagcttc 2401 tcaccatctc tgagaagcct gtcgggcgcc ccctgcaggc tgtcccgggc agagtccgag 2461 cgcagatgtc gtgcacctgg gcagccccca gggagcgctc tgtgccatga ccgtggccgg 2521 tgcgagtgtg gggtgtgcat ctgtcacgtg actgaacctg gcacctactt tgggccactg 2581 tgtgagtgcc atgactgggt ctgcgagacc tacgacggga aaacctgtgc aggccacggt 2641 acttgtgact gcggcaagtg taagtgtgat gtgggatggt ctggggaagc ttgccagtac 2701 ccgaccaagt gcgacctgac caaaaaaata agcaaccaga tgtgcaagaa ctctcaagat 2761 gtcatctgct ccaacgcagg tacatgtcac tgtggcaggt gtaagtgtga taattcagat 2821 ggacatggac tcatttacgg taaattttgt gagtgtgatg atagagaatg catagatgat 2881 gaaacagaag aaatatgtgg aggccatggg aagtgttact gtggaaactg ttactgcgag 2941 gctggttggc atggagataa atgcgagttc cagtgtgaca tcaccccctg ggaaagcaag 3001 cgaagatgca catctccaga tggcaaagtc tgtagcaaca gaggaacatg tgtatgtggt 3061 gaatgttctt gccatgatgt tgatccaact ggcgactggg gagacattca tggagacaca 3121 tgcgagtgtg atgaaaggga ctgcagagct gtttatgatc gatactctga tgatttctgt 3181 tcaggtcacg ggcagtgtaa ctgtggaaga tgtgactgca gagcaggctg gtatgggaag 3241 aagtgtgagc acccaaagaa ctgcccattg tcagctgagg agagcaccag aaagtgccag 3301 ggtagctctg atctgccttg ttctggaagg ggcagatgcg aatgtggcag atgtacctgt 3361 taccctcccg gggacagcag agtatatggc aagacctgtg agtgtgatga ccggcgctgt 3421 gaagacctag acggtgtggt ctgtggaggc cgtggcacgt gctcctgtgg tcgttgtgtt 3481 tgtgagaaag gatggtttgg taagctctgc caacaccctc ggaagtgtaa tatgaccgaa 3541 gaacaaagca ggagtctgtg tgagtcagca gatggcacat tgtgctcggg gaagggttct 3601 tgtcattgtg gaaagtgcat ttgttctgga gaagagtggt atatttcagg ggagttttgt 3661 gactgtgatg acagagactg tgacaaacac gacggtctca tttgcacagg gaatggaatc 3721 tgtagctgtg gaaactgtga atgctgggat ggatggaatg gaaatgcatg tgaaatctgg 3781 cttggtaccg aatatcctgg atccGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT 3841 GACATCGACT ACAAGGATGA CGATGACAAG tagcggccgc Gaaggatctg cgatcgctcc 3901 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 3961 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 4021 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 4081 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 4141 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg 4201 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta 4261 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga 4321 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg 4381 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc 4441 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 4501 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 4561 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 4621 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 4681 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 4741 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4801 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4861 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4921 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4981 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 5041 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 5101 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 5161 gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag tcttctaaca 5221 tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac ggtgcgcctc 5281 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 5341 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 5401 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5461 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5521 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5581 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5641 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5701 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5761 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5821 tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa aatttgtgaa 5881 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5941 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6001 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6061 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6121 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6181 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6241 gggaaATCAT CGTCCTTTCC Ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 6301 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6361 ctgccggctc tgcggcctct tccgcgtctT cgccttcgcc ctcagacgag tcggatctcc 6421 ctttggGccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga 6481 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG 6541 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 6601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 6661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 6721 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 6781 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 6841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 6901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctaTC 6961 CCGCCCCTAA CTCCGCCCAt cccgccccta actccgccca gttccgccca ttctccgccc 7021 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7081 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt 7141 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 7201 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 7261 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 7321 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 7381 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 7441 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 7501 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 7561 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7621 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7681 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7741 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7801 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7861 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7921 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7981 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 8041 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 8101 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 8161 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 8221 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 8281 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 8341 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 8401 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 8461 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 8521 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 8581 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 8641 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8701 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8761 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8821 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8881 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 8941 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 9001 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 9061 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 9121 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 9181 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 9241 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 9301 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 9361 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 9421 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 9481 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 9541 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 9601 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 9661 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 9721 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.