pCMV-T7-3×FLAG-ATG9A-Neo plasm

pCMV-T7-3×FLAG-ATG9A-Neo plasmid

PVT87746

pCMV-T7-3×FLAG-ATG9A-Neo plasmid Information

Plasmid name: pCMV-T7-3×FLAG-ATG9A(human)-Neo
Alias: NM_001077198.3;APG9L1; mATG9; MGD3208
Gene lenght: 2517bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-3×FLAG-MCS-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-T7-3×FLAG-ATG9A-Neo plasmid Description

pCMV-T7-3×FLAG-ATG9A-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate efficient expression of the human ATG9A gene, promoting the N-terminal fusion expression of the ATG9A gene with a 3×FLAG tag, facilitating detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid carries a Neo/G418 resistance gene for selecting host cells expressing the target gene.

pCMV-T7-3×FLAG-ATG9A-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggcgcagt ttgacactga ataccagcgc 1021 ctagaggcct cctatagtga ttcaccccca ggggaggagg acctgttggt gcacgtcgcc 1081 gaggggagca agtcaccttg gcaccatatt gaaaaccttg acctcttctt ctctcgagtt 1141 tataatctgc accagaagaa tggcttcaca tgtatgctca tcggggagat ctttgagctc 1201 atgcagttcc tctttgtggt tgccttcact accttcctgg tcagctgcgt ggactatgac 1261 atcctatttg ccaacaagat ggtgaaccac agtcttcacc ctactgaacc cgtcaaggtc 1321 actctgccag acgccttttt gcctgctcaa gtctgtagtg ccaggattca ggaaaatggc 1381 tcccttatca ccatcctggt cattgctggt gtcttctgga tccaccggct tatcaagttc 1441 atctataaca tttgctgcta ctgggagatc cactccttct acctgcacgc tctgcgcatc 1501 cctatgtctg cccttccgta ttgcacgtgg caagaagtgc aggcccggat cgtgcagacg 1561 cagaaggagc accagatctg catccacaaa cgtgagctga cagaactgga catctaccac 1621 cgcatcctcc gtttccagaa ctacatggtg gcactggtta acaaatccct cctgcctctg 1681 cgcttccgcc tgcctggcct cggggaagct gtcttcttca cccgtggtct caagtacaac 1741 tttgagctga tcctcttctg gggacctggc tctctgtttc tcaatgaatg gagcctcaag 1801 gccgagtaca aacgtggggg gcaacggcta gagctggccc agcgcctcag caaccgcatc 1861 ctgtggattg gcatcgctaa cttcctgctg tgccccctca tcctcatatg gcaaatcctc 1921 tatgccttct tcagctatgc tgaggtgctg aagcgggagc cgggggccct gggagcacgc 1981 tgctggtcac tctatggccg ctgctacctc cgccacttca acgagctgga gcacgagctg 2041 cagtcccgcc tcaaccgtgg ctacaagccc gcctccaagt acatgaattg cttcttgtca 2101 cctcttttga cactgctggc caagaatgga gccttcttcg ctggctccat cctggctgtg 2161 cttattgccc tcaccattta tgacgaagat gtgttggctg tggaacatgt gctgaccacc 2221 gtcacactcc tgggggtcac cgtgaccgtg tgcaggtcct ttatcccgga ccagcacatg 2281 gtgttctgcc ctgagcagct gctccgcgtg atcctcgctc acatccacta catgcctgac 2341 cactggcagg gtaatgccca ccgctcgcag acccgggacg agtttgccca gctcttccag 2401 tacaaggcag tgttcatttt ggaagagttg ctgagcccca ttgtcacacc cctcatcctc 2461 atcttctgcc tgcgcccacg ggccctggag attatagact tcttccgaaa cttcaccgtg 2521 gaggtcgttg gtgtgggaga tacctgctcc tttgctcaga tggatgttcg ccagcatggt 2581 catccccagt ggctatctgc tgggcagaca gaggcctcag tgtaccagca agctgaggat 2641 ggaaagacag agttgtcact catgcacttt gccatcacca accctggctg gcagccacca 2701 cgtgagagca cagccttcct aggcttcctc aaggagcagg ttcagcggga tggagcagct 2761 gctagcctcg cccaaggggg tctgctccct gaaaatgccc tctttacgtc tatccagtcc 2821 ttacaatctg agtctgagcc cctgagcctt atcgcaaatg tggtagctgg ctcatcctgc 2881 cggggccctc cactgcccag agacctgcag ggctccaggc acagggctga agtcgcctct 2941 gccctgcgct ccttctcccc gctgcaaccc gggcaggcgc ccacaggccg ggctcacagc 3001 accatgacag gctctggggt ggatgccagg acagccagct ccgggagcag cgtgtgggaa 3061 ggacagctgc agagcctggt gctgtcagaa tatgcatcca cagagatgag cctgcatgcc 3121 ctctatatgc accagctcca caagcagcag gcccaggctg aacctgagcg gcatgtatgg 3181 caccgccggg agagtgatga gagtggagaa agcgcccctg atgaaggggg agagggcgcc 3241 cgggcccccc agtctatccc tcgctctgct agctatccct gtgcagcacc ccggcctgga 3301 gctcctgaga ccactgccct gcatgggggc ttccagaggc gctacggtgg catcacagat 3361 cctggcacag tgcccagggt tccctctcat ttctctcggc tgcctcttgg agggtgggca 3421 gaagatgggc agtcggcatc aaggcaccct gagcccgtgc ccgaagaggg ctcggaggat 3481 gagctacccc ctcaggtgca caaggtaTAA gaattctgca gatatccagc acagtggcgg 3541 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt 3601 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 3661 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 3721 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 3781 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 3841 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 3901 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 3961 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 4021 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 4081 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 4141 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 4201 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 4261 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 4321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 4381 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 4441 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 4501 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 4561 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 4621 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 4681 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 4741 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 4801 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 4861 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 4921 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 4981 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 5041 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 5101 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 5161 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 5221 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 5281 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 5341 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 5401 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 5461 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 5521 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 5581 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 5641 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 5701 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5761 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 5821 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 5881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 5941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 6001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 6061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 6121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 6181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt 6781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6961 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7021 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7081 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7141 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7201 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7261 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 7321 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 7381 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 7441 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7501 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7561 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7621 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7681 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7741 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7801 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 7861 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7921 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7981 tgacgtc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.