pLV3-CMV-NDUFA11-3×FLAG-CopGFP

pLV3-CMV-NDUFA11-3×FLAG-CopGFP-Puro plasmid

PVT87652

pLV3-CMV-NDUFA11-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-NDUFA11(human)-3×FLAG-CopGFP-Puro
Alias: NM_001193375.3;B14.7; CI-B14.7; MC1DN14
Gene lenght: 684bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-NDUFA11-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-NDUFA11-3×FLAG-CopGFP-Puro This is a low-copy plasmid suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate transcription of the human NDUFA11 gene, promoting the C-terminal fusion expression of a 3×FLAG tag, which facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid also carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, allowing for stable integration of the gene into host cells. The plasmid includes CopGFP green fluorescent protein to detect the expression and localization of the target gene. Additionally, the plasmid carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-NDUFA11-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgccgaaggt ttttcgtcag 2341 tactgggata tccccgatgg caccgattgc caccgcaaag cctacagcac caccagtatt 2401 gccagcgtcg ctggcctgac cgccgctgcc tacagagtca cactcaatcc tccgggcacc 2461 ttccttgaag gagtggctaa ggttggacaa tacacgttca ctgcagctgc tgtcggggcc 2521 gtgtttggcc tcaccacctg catcagcgcc catgtccgcg agaagcccga cgaccccctg 2581 aactacttcc tcggtggctg cgccggaggc ctgactctgg gagcacgcaa gacaggatct 2641 cactgtgttg tgcaggctgg tctcaaactc ctggcctcga gcagtcctca cacctcagcc 2701 tcccagagtg ctgggattat aggcatgagc cactgtgtac agagattctg ggtcccttct 2761 tccagcgcct gcctcgaagt cctctctgga gagagcacag atgtccacgc gtgttccagc 2821 acaagggggg catgcaacag ctcagggtca cgtccacttc cggagctggg tgcccgggcc 2881 tcgggctccc tgagaaaggg cggacacacc caccccgcac ccagaggggc aggggccctg 2941 acacctgtcc aagccctaat agagtcattg ttaaacactc tgggttcgaa ccccagaact 3001 ggatccGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 3061 GACGATGACA AGtagcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca 3121 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt 3181 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt 3241 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt 3301 cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca 3361 cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct 3421 cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg 3481 agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg 3541 ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt 3601 tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag cgacgagagc 3661 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc 3721 gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa caagatgaag 3781 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc 3841 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac 3901 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg 3961 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt ggtgggcacc 4021 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg 4081 gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg caccttcagc 4141 ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt caagagcgcc 4201 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag 4261 ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gacccccatc 4321 gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac cgccggaccc 4381 ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga cgtggaggag 4441 aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 4501 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 4561 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 4621 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 4681 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 4741 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 4801 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 4861 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 4921 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 4981 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 5041 cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 5101 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 5161 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 5221 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 5281 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 5341 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 5401 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaATC ATCGTCCTTT 5461 CCTtggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 5521 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 5581 cttccgcgtc tTcgccttcg ccctcagacg agtcggatct ccctttggGc cgcctccccg 5641 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa 5701 agaaaagggg ggactggaag ggctaattca ctcccaacga aGataagatc tgctttttgc 5761 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5821 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5881 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5941 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6001 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6061 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6121 caaactcatc aatgtatctt atcatgtctg gctctagcta TCCCGCCCCT AACTCCGCCC 6181 Atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6301 ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt catagctgtt 6361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 7201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7621 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 7801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 8521 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt 8581 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct 8641 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg 8701 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 8761 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt 8821 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 8881 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc 8941 acgacgttgt aaaacgacgg ccagtgccaa gctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.