pLV3-CMV-FZD3-3×FLAG-CopGFP-Pu

pLV3-CMV-FZD3-3×FLAG-CopGFP-Puro plasmid

PVT87627

pLV3-CMV-FZD3-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-FZD3(human-toxic-opt)-3×FLAG-CopGFP-Puro
Alias: NM_017412.4;Fz-3;
Gene lenght: 1998bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FZD3-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-FZD3-3×FLAG-CopGFP-Puro This plasmid is suitable for low-copy overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate transcription of the human FZD3 gene, promoting the C-terminal fusion expression of the FZD3 gene with a 3×FLAG tag, facilitating detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating stable integration of the gene into host cells. The plasmid carries CopGFP green fluorescent protein for detecting the expression and localization of the target gene. The plasmid also carries a Puro resistance gene for selecting host cells expressing the target gene.

pLV3-CMV-FZD3-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGG CTATGACATG GATAGTATTT 2341 TCACTTTGGC CTCTCACAGT GTTCATGGGC CACATAGGAG GGCATTCCCT GTTCTCATGT 2401 GAACCCATCA CCCTTCGGAT GTGCCAGGAC CTGCCGTATA ATACCACCTT CATGCCGAAC 2461 TTGCTCAACC ATTACGATCA ACAGACTGCA GCCCTCGCAA TGGAACCCTT TCACCCGATG 2521 GTCAACTTGG ATTGTTCCCG AGACTTTAGG CCCTTTTTGT GCGCTCTGTA CGCTCCGATT 2581 TGCATGGAGT ATGGGAGAGT CACTCTTCCT TGTCGCAGAC TCTGTCAGCG CGCCTACTCA 2641 GAATGTAGCA AACTCATGGA GATGTTCGGG GTGCCCTGGC CAGAGGATAT GGAGTGCTCT 2701 CGCTTTCCAG ATTGTGACGA GCCCTATCCC AGGCTGGTTG ATCTGAATCT GGCCGGCGAG 2761 CCAACAGAGG GTGCCCCAGT GGCCGTGCAG AGGGATTACG GATTCTGGTG CCCAAGGGAG 2821 CTTAAGATCG ATCCCGATCT GGGATACTCA TTTTTGCATG TTAGGGACTG CAGCCCCCCT 2881 TGTCCTAATA TGTATTTCAG ACGGGAAGAG CTCAGCTTTG CCCGGTATTT CATCGGGCTC 2941 ATTTCCATCA TCTGTCTGTC AGCTACACTG TTCACGTTTT TGACTTTCCT GATTGACGTG 3001 ACTCGATTCC GCTATCCCGA AAGGCCAATC ATTTTTTACG CCGTGTGTTA CATGATGGTC 3061 AGTCTGATCT TTTTTATCGG CTTTCTGCTC GAGGACAGGG TCGCTTGCAA CGCCAGCATC 3121 CCTGCTCAAT ACAAAGCATC CACCGTGACC CAGGGAAGCC ATAATAAGGC TTGTACCATG 3181 CTCTTTATGA TCCTGTACTT TTTCACCATG GCTGGTAGCG TGTGGTGGGT GATTCTGACC 3241 ATCACCTGGT TTCTGGCCGC TGTGCCAAAG TGGGGATCCG AGGCAATTGA GAAGAAAGCA 3301 CTGCTCTTCC ATGCCAGCGC TTGGGGGATC CCAGGGACCC TGACGATTAT CCTCTTGGCA 3361 ATGAACAAGA TCGAGGGTGA TAATATTTCT GGAGTGTGTT TTGTGGGACT GTACGACGTC 3421 GACGCACTGC GATATTTTGT GCTGGCTCCT CTGTGCCTGT ATGTCGTGGT GGGGGTATCC 3481 CTGCTTCTCG CTGGCATTAT CTCTCTGAAC CGAGTCAGGA TCGAAATCCC ATTGGAAAAA 3541 GAGAATCAGG ACAAGCTGGT GAAATTTatg atccggatcg gtgttttcag cattctttat 3601 ctcgtaccac tcttggttgt aattggatgc tacttttatg agcaagctta ccggggcatc 3661 tgggaaacaa cgtggataca agaacgctgc agagaatatc acattccatg tccatatcag 3721 gttactcaaa tgagtcgtcc agacttgatt ctctttctga tgaaatacct gatggctctc 3781 atagttggca ttccctctgt attttgggtt ggaagcaaaa agacatgctt tgaatgggcc 3841 agtttttttc atggtcgtag gaaaaaagag atagtgaatg agagccgaca ggtactccag 3901 gaacctgatt ttgctcagtc tctcctgagg gatccaaata ctcctatcat aagaaagtca 3961 aggggaactt ccactcaagg aacatccacc catgcttctt caactcagct ggctatggtg 4021 gatgatcaaa gaagcaaagc aggaagcatc cacagcaaag tgagcagcta ccacggcagc 4081 ctccacagat cacgtgatgg caggtacacg ccctgcagtt acagaggaat ggaggagaga 4141 ctacctcatg gcagcatgtc acgactaaca gatcactcca ggcatagtag ttctcatcgg 4201 ctcaatgaac agtcacgaca tagcagcatc agagatctca gtaataatcc catgactcat 4261 atcacacatg gcaccagcat gaatcgggtt attgaagaag atggaaccag tgctggatcc 4321 GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT 4381 GACAAGtagc ggccgcGaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc 4441 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag 4501 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc 4561 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac 4621 gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc 4681 cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc 4741 tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg 4801 ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc 4861 ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga 4921 tccaagctgt gaccggcgcc tacgctagac gccaccatgg agagcgacga gagcggcctg 4981 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg 5041 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc 5101 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac 5161 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc 5221 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc 5281 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc 5341 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac 5401 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc 5461 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac 5521 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac 5581 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc 5641 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc 5701 accggatctc gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc 5761 ggccctatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg 5821 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat 5881 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5941 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 6001 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 6061 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 6121 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 6181 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 6241 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 6301 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 6361 gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6421 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 6481 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 6541 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 6601 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 6661 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 6721 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aATCATCGTC CTTTCCTtgg 6781 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 6841 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 6901 cgtctTcgcc ttcgccctca gacgagtcgg atctcccttt ggGccgcctc cccgcctggt 6961 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 7021 ggggggactg gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac 7081 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 7141 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 7201 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 7261 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 7321 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 7381 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 7441 catcaatgta tcttatcatg tctggctcta gctaTCCCGC CCCTAACTCC GCCCAtcccg 7501 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 7561 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 7621 tttggaggcc tagacttttg cagagaccaa attcgtaatc atgtcatagc tgtttcctgt 7681 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7741 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7801 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 8041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 8101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 8161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 8221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 8281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 8341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 8401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 8461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 8521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 8581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 9001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 9061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 9121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 9181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 9241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 9301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 9361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 9421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 9481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 9541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9781 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9841 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9901 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9961 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 10021 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 10081 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 10141 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 10201 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 10261 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.