pLV3-CMV-3×HA-PDZD11-CopGFP-Pu

pLV3-CMV-3×HA-PDZD11-CopGFP-Puro plasmid

PVT87566

pLV3-CMV-3×HA-PDZD11-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×HA-PDZD11(human)-CopGFP-Puro
Alias: NM_016484.5;AIPP1; PDZK11; PISP
Gene lenght: 420bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-3×HA-MCS-EF1a-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×HA-PDZD11-CopGFP-Puro plasmid Description

pLV3-CMV-3×HA-PDZD11-CopGFP-Puro This plasmid is suitable for gene overexpression in mammalian cells. The strong CMV promoter on the plasmid can initiate the transcription of the human PDZD11 gene, promoting the N-terminal fusion expression of the PDZD11 gene with a 3×HA tag, facilitating experiments such as protein immunoprecipitation and co-immunoprecipitation. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentiviral packaging, facilitating the stable integration of the gene into host cells. The plasmid carries the CopGFP green fluorescent protein for observing the expression and localization of the target gene. The plasmid carries a Puro resistance gene for screening host cells expressing the target gene.

pLV3-CMV-3×HA-PDZD11-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatgTAC CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG TACCTGATTA 2341 TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCAgaattc atggacagcc ggattcctta 2401 tgatgactac ccggtggttt tcttgcctgc ctatgagaat cctccagcat ggattcctcc 2461 tcatgagagg gtacaccacc cggactacaa caatgagttg acccagtttc tgccccgaac 2521 catcacactg aagaagcctc ctggagctca gttgggattt aacatccgag gaggaaaggc 2581 ctcccagcta ggcatcttca tctccaaggt gattcctgac tctgatgcac atagagcagg 2641 actgcaggaa ggggaccaag ttctagctgt gaatgatgtg gatttccaag atattgagca 2701 cagcaaggct gttgagatcc tgaagacagc tcgtgaaatc agcatgcgtg tgcgcttctt 2761 tccctacaat tatcatcgcc aaaaagagag gactgtgcac TAGggatccg cggccgcgaa 2821 ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg 2881 agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa 2941 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt 3001 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac 3061 agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc 3121 gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg 3181 cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc 3241 cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac 3301 tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc 3361 ctacgctaga cgccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt 3421 gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca 3481 cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca 3541 gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca 3601 gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca 3661 tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg 3721 gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct 3781 tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata 3841 acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct 3901 tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg 3961 ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca 4021 tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt 4081 cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct cgcgagggca 4141 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg accgagtaca 4201 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 4261 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 4321 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 4381 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 4441 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 4501 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 4561 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 4621 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 4681 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 4741 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg 4801 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat 4861 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 4921 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca 4981 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 5041 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 5101 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca 5161 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 5221 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 5281 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc cttcgccctc 5341 agacgagtcg gatctccctt tggccgcctc cccgcctggt acctttaaga ccaatgactt 5401 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 5461 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 5521 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 5581 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 5641 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 5701 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 5881 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 5941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 6001 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta 6061 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 6121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 6181 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 6241 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 6301 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6361 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6421 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6481 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 6541 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 6601 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 6661 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 6721 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 6781 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 6841 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 6901 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 6961 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7021 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7081 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7141 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7201 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7261 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7321 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7381 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7441 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7501 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 7561 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 7621 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 7681 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 7741 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 7801 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 7861 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 7921 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 7981 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8041 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8101 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8161 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8281 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 8341 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 8401 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 8461 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 8521 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 8581 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 8641 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.