pCMV-Ccdc80-3×FLAG-mCMV-EGFP p

pCMV-Ccdc80-3×FLAG-mCMV-EGFP plasmid

PVT96149

pCMV-Ccdc80-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-Ccdc80(mouse)-3×FLAG-mCMV-EGFP
Alias: NM_026439.3;2610001E17Rik; DRO1; Ssg1; Urb
Gene lenght: 2850bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ccdc80-3×FLAG-mCMV-EGFP plasmid Description

pCMV-Ccdc80-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-Ccdc80-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatgatg tggaaaatgg gaccccactt 661 caccacgctc ttggccatgt ggctggtgtg tggctcagca tctcattctc ctgccttgga 721 tagcgacagc cacactggac ggaaagtacc tttggtttct ccaatcagca gtaggtcagc 781 tcggtatctg aggcacaccg ggaggtctgg tggagttgag aaatccaccc aggaagaacc 841 aaatcctcag cctttccaaa ggaggaagag tgtaccagtg ttgagattag ctcacccgac 901 catgagacca cccccctcag gtatcaatgg agttccagtc agacctgagg tgagacccat 961 agctaggagc tcggcgcgtg agatggtccg agatgagggg tcttctgctc ggacaagaat 1021 gttgcgattc ccctctggat ctagctctcc caatatcctg gccagctttg caggaaagaa 1081 cagggtatgg gtcatctcag cccctcacgc ctcagagggc tactaccgcc tcatgatgag 1141 cctcctgaag gacgacgtgt attgtgagct ggcagaaagg cacatccaac agattgtgct 1201 cttccaccag gcaggcgagg aagggggcaa ggtacggagg atcaccaatg aagggcagat 1261 cctggagcag cctctggacc ccaatctcat cccaaagctg atgagcttct tgaaactgga 1321 gaagggcaag tttagcatgg tgctgttaaa aaagactctg caggtggagg agcgctaccc 1381 ttacccggtc aggctggaag ccatgtatga ggtcattgat caaggtccca tccgcaggat 1441 cgagaaaatc aggcagaagg gttttgtcca aaagtgtaag gcctcgggca tagagggcca 1501 tgtagtccag gaaggaaacg agggcggtgg aggagcagga ggtacaggcc tgggcggtga 1561 taagaggaaa gaggacccaa ggagaacaca agtccacccc accagagagg ctccgagaaa 1621 gcaggccacc agcaaggcag ccactcctca gcctccccca actccaaggg ccaccaccct 1681 tcctcctgct ccagtcacga cagccactcg ggccacatcc cgggtagtga caatagccgc 1741 aagacctaca accaccactg cctatccagc tacccagagg ccctggacat cccggttgca 1801 tcccttctca gtctcccaca ggccccctgc aacagcagag gtgaccactg ccaggggccc 1861 ctccgtctca gagcagctat accctctacc tcggaaggag caacagagag agaagccaca 1921 ggccaccagg aggcccagca aagccaccaa ctatggaagc ttcacagcca ccccacctcc 1981 caccctctgg gaggtcagcg caagagttgt gggcacaagc cgtttccggg acaaccggac 2041 agacaaacgg gaacatggcc atcaggaccc aaatgcggtg ccaggtcctc acaagccagt 2101 aaaggggaag ctgcccaaaa agaaggacag aattctcagc aacgaatatg aagacaagta 2161 tgacctcagc cagcccacct cctctcaggg ggaggaggag cggcaggtgg atagcgttcc 2221 ctcgcagaat gccaaggagt cgaaaaagct tgaaaagctc gagaaacctg agaaggagaa 2281 gaagaaaaaa gggaagagtg caaaacaaga caagttactc aagagtgaaa agcaagcgaa 2341 gaaagctgag aaaaagacca aacaggagaa agacaagaat aagaagaaaa aggcgggtaa 2401 gacagagcag gatgacaatc agaagcccac cgcaaaacat ctcgctccaa gtcccaagaa 2461 gtcagtggcc gacctgttgg ggtctttcga aggcaaacga agactcctcc tgatcaccac 2521 tcccaaggct gagaacaata tgtatgtgca gcagcgggat gaatatctgg agagcttctg 2581 caagatggcc accaggagga tctctgtggt caccatcttt ggtcctgtca acaacagctc 2641 catgaaaatt gatcacttcc agctagataa tgagaaaccc atgcgtgtgg tggatgatga 2701 tgacttggtg gaccaacatc tcatcagcga gctgaggaag gagtatggaa tgacctacga 2761 tgacttcttc atggtgctga cagacgtgga tctgagagtc aagcaatact acgaagtgcc 2821 aatagcaatg aagtccgtgt ttgatcttat tgacactttc caatcccgaa tcaaagatat 2881 ggagaagcag aagaaggagg gcattgcctg caaggaggac aagaggcagt ccctggagaa 2941 cttcctatcc aggttccgat ggaggaggag gttgctggtg atctctgctc ctaatgatga 3001 agactgggcc tattcacaac agctctctgc cctcaacggt caggcatgca attttggtct 3061 gcgacatata accattctga agcttttggg tgttggagag gaagttggag gagttttaga 3121 actgttccca attaatggga gctccatcgt tgagcgggaa gatgtgccag cccacttggt 3181 caaagatatc cgcaactatt ttcaagtgag cccagagtac ttctccatgc ttctagttgg 3241 aaaagatggc aatgttaaat cttggtatcc ttctcctatg tggtcgatgg tcatcgtgta 3301 tgacttaatt gattccatgc aacttcggag acaggaaatg gccattcagc agtctctggg 3361 gatgcgctgc ccagaagatg agtatgcagg atatggttac catagttatc accaaggata 3421 ccaggatggc taccaggatg actaccgtca tcatgaaagt taccaccatg gataccctta 3481 cAAGCTTACG CGTGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA 3541 CAAGGATGAC GATGACAAGT GAGGATCCGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC 3601 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT 3661 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT 3721 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG 3781 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGTCTAGA GATATACTGA 3841 GTCATTAGGG ACTTTCCAAT GGGTTTTGCC CAGTACATAA GGTCAATAGG GGTGAATCAA 3901 CAGGAAAGTC CCATTGGAGC CAAGTACACT GAGTCAATAG GGACTTTCCA TTGGGTTTTG 3961 CCCAGTACAA AAGGTCAATA GGGGGTGAGT CAATGGGTTT TTCCCATTAT TGGCACGTAC 4021 ATAAGGTCAA TAGGGGTGAG TCATTGGGTT TTTCCAGCCA aTTTAATTAA AACGCCATGT 4081 ACTTTCCCAC CATTGACGTC AATGGGCTAT TGAAACTAAT GCAACGTGAC CTTTAAACGG 4141 TACTTTCCCA TAGCTGATTA ATGGGAAAGT ACCGTTCTCG AGCCAATACA CGTCAATGGG 4201 AAGTGAAAGG GCAGCCAAAA CGTAACACCG CCCCGGTTTT CCCCTGGAAA TTCCATATTG 4261 GCACGCATTC TATTGGCTGA GCTGCGTTCT ACGTGGGTAT AAGAGGCGCG ACCAGCGTCG 4321 GTACCGTCGC AGTCTTCGGT CTGACCACCG TAGAACGCAG ATCaccggtc gccaccatgg 4381 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 4441 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 4501 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 4561 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 4621 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 4681 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 4741 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4801 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 4861 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 4921 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 4981 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 5041 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 5101 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 5161 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 5221 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 5281 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 5341 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 5401 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 5461 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 5521 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 5581 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 5641 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 5701 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 5761 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 5821 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 5881 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 5941 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 6001 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 6061 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 6121 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 6181 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 6241 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 6301 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 6361 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 6421 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 6481 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 6541 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 6601 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 6661 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 6721 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 6781 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 6841 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 6901 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 6961 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 7021 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 7081 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 7141 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 7201 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 7261 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 7321 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 7381 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 7441 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 7501 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 7561 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 7621 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 7681 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 7741 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 7801 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 7861 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 7921 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 7981 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 8041 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 8101 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 8161 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 8221 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 8281 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 8341 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 8401 ttctgtggat aaccgtatta ccgccatgca t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.