pLV2-U6-PDSS1-shRNA1-EGFP-Puro

pLV2-U6-PDSS1-shRNA1-EGFP-Puro plasmid

PVT87560

pLV2-U6-PDSS1-shRNA1-EGFP-Puro plasmid Information

Plasmid name: pLV2-U6-PDSS1(human)-shRNA1-EGFP-Puro
Alias: GCACGAATTGGAAATACAA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLVshRNA-EGFP(2A)Puro
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV2-U6-PDSS1-shRNA1-EGFP-Puro plasmid Description

pLV2-U6-PDSS1-shRNA1-EGFP-Puro This plasmid is suitable for gene knockdown in mammalian cells. The U6 promoter on the plasmid can initiate the transcription of shRNA fragments designed against the human PDSS1 gene, reducing the expression of the PDSS1 gene. Additionally, the plasmid contains HIV-1 Ψ and 3'/5' LTR elements for lentiviral packaging, facilitating the stable integration of the interference fragments into host cells. The plasmid carries the EGFP green fluorescent protein for visual observation of the expression and localization of the target gene.

pLV2-U6-PDSS1-shRNA1-EGFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATCTGGGC AGGAAGAGGG CCTATTTCCC ATGATTCCTT 2221 CATATTTGCA TATACGATAC AAGGCTGTTA GAGAGATAAT TAGAATTAAT TTGACTGTAA 2281 ACACAAAGAT ATTAGTACAA AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG 2341 CAGTTTTAAA ATTATGTTTT AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT 2401 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG ATCCGGCACG AATTGGAAAT 2461 ACAAttcaag agaTTGTATT TCCAATTCGT GCTTTTTTGA ATTCTACCGG GTAGGGGAGG 2521 CGCTTTTCCC AAGGCAGTCT GGAGCATGCG CTTTAGCAGC CCCGCTGGGC ACTTGGCGCT 2581 ACACAAGTGG CCTCTGGCCT CGCACACATT CCACATCCAC CGGTAGGCGC CAACCGGCTC 2641 CGTTCTTTGG TGGCCCCTTC GCGCCACCTT CTACTCCTCC CCTAGTCAGG AAGTTCCCCC 2701 CCGCCCCGCA GCTCGCGTCG TGCAGGACGT GACAAATGGA AGTAGCACGT CTCACTAGTC 2761 TCGTGCAGAT GGACAGCACC GCTGAGCAAT GGAAGCGGGT AGGCCTTTGG GGCAGCGGCC 2821 AATAGCAGCT TTGCTCCTTC GCTTTCTGGG CTCAGAGGCT GGGAAGGGGT GGGTCCGGGG 2881 GCGGGCTCAG GGGCGGGCTC AGGGGCGGGG CGGGCGCCCG AAGGTCCTCC GGAGGCCCGG 2941 CATTCTGCAC GCTTCAAAAG CGCACGTCTG CCGCGCTGTT CTCCTCTTCC TCATCTCCGG 3001 GCCTTTCGAC CTGCAGCCCA AGCTTACCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT 3061 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG 3121 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG 3181 CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT 3241 GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT 3301 GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC 3361 CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT 3421 CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA 3481 CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG 3541 CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT 3601 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG 3661 CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG 3721 GATCACTCTC GGCATGGACG AGCTGTACAA GGAATTTGGC AGCGGCCAGT GCACCAACTA 3781 TGCCCTGCTG AAACTGGCTG GCGATGTGGA AAGCAACCCC GGACCCATGA CCGAGTACAA 3841 GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA CCCTCGCCGC 3901 CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC ACATCGAGCG 3961 GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG 4021 GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG 4081 GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC 4141 GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT 4201 GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT 4261 CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC 4321 CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC 4381 CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC CGCCCCACGA 4441 CCCGCAGCGC CCGACCGAAA GGAGCGCACG ACCCCATGCA TCGGCGTCTC GAGTCTAGAG 4501 GGCCCGACGC GTCTGGAACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 4561 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGGCTTTGTA 4621 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 4681 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 4741 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 4801 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 4861 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC 4921 GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 4981 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 5041 GCGGCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 5101 CCCGCCTGGA ATTAATTCTG CAGTCGAGAC CTAGAAAAAC ATGGAGCAAT CACAAGTAGC 5161 AATACAGCAG CTATCAATGA TTGTGCCTGG CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT 5221 TTTCCAGTCA CACCTCAGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT 5281 AGCCACTTTT TAAAAGAAAA GAGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGACAA 5341 GATATCCTTG ATCTGTGGAT CTACCACACA CAAGGCTACT TCCCTGATTA GCAGAACTAC 5401 ACACCAGGGC CAGGGGTCAG ATATCCACTG ACCTTTGGAT GGTGCTACAA GCTAGTACCA 5461 GTTGAGCCAG ATAAGGTAGA AGAGGCCAAT AAAGGAGAGA ACACCAGCTT GTTACACCCT 5521 GTGAGCCTGC ATGGGATGGA TGACCCGGAG AGAGAAGTGT TAGAGTGGAG GTTTGACAGC 5581 CGCCTAGCAT TTCATCACGT GGCCCGAGAG CTGCATCCGG AGTACTTCAA GAACTGCTGA 5641 TATCGAGCTT GCTACAAGGG ACTTTCCGCT GGGGACTTTC CAGGGAGGCG TGGCCTGGGC 5701 GGGACTGGGG AGTGGCGAGC CCTCAGATCC TGCATATAAG CAGCTGCTTT TTGCCTGTAC 5761 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 5821 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 5881 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 5941 CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC 6001 AGAGAGTGAG AGGCCTTGAC ATTGCTAGCG TTTACCGTCG ACCTCTAGCT AGAGCTTGGC 6061 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 6121 CATACGAGCC GGAAGCATAA AGTGTAAAgc ctggggtgcc taatgagtga gctaactcac 6181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 6361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 6421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 6481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 6541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 6601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 6661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 6721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 6781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 6841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 6901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 6961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 7021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 7081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 7141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 7201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 7261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 7321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 7381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 7441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 7561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 7621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 7681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 8041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 8101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 8161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 8221 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa 8281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 8341 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc 8401 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc 8461 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt 8521 aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac 8581 aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.