pCMV-SMG5-3×HA-Neo plasmid

pCMV-SMG5-3×HA-Neo plasmid

PVT87479

pCMV-SMG5-3×HA-Neo plasmid Information

Plasmid name: pCMV-SMG5(human)-3×HA-Neo
Alias: NM_001323617.2;EST1B; LPTS-RP1; LPTSRP1; SMG-5
Gene lenght: 2850bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-3×HA-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SMG5-3×HA-Neo plasmid Description

pCMV-SMG5-3×HA-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the human SMG5 gene, promoting the C-terminal fusion expression of a 3×HA tag, which facilitates experiments such as protein immunoprecipitation and co-immunoprecipitation. The plasmid contains a Neo/G418 resistance gene for screening host cells expressing the target gene.

pCMV-SMG5-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgtt cctgcaccca 721 gtggactatg ggagaaaggc tgaggagctg ctgtggagaa aggtatacta tgaagttatc 781 cagcttatca agactaacaa aaagcacatc cacagccgga gcactttgga atgtgcctac 841 aggacgcacc tggttgctgg tattggcttc taccagcatc tccttctcta tatccagtcc 901 cactaccagc tggaactgca gtgctgcatc gactggaccc atgtcactga ccccctcata 961 ggatgcaaga agccagtgtc tgcctcaggg aaggagatgg attgggcaca gatggcatgt 1021 caccgatgtc tggtgtatct gggggatttg tcccgatatc agaatgaatt agctggcgta 1081 gataccgagc tgctagccga gagattttac taccaagccc tgtcagtagc tcctcagatt 1141 ggaatgccct tcaatcagct gggcaccctg gcaggcagca agtactataa tgtggaagcc 1201 atgtattgct acctgcgctg catccagtca gaagtgtcct ttgagggagc ctatgggaac 1261 ctcaagcggc tgtatgacaa ggcagccaaa atgtaccacc aactgaagaa gtgtgagact 1321 cggaaactgt ctcctggcaa aaagcgatgt aaagacatta aaaggttgct agtgaacttt 1381 atgtatctgc aaagcctcct acagcccaaa agcagctccg tggactcaga gctgacctca 1441 ctttgccagt cagtcctgga ggacttcaac ctctgcctct tctacctgcc ctcctcaccc 1501 aacctcagcc tggccagtga ggatgaggag gagtatgaga gtggatatgc tttcctcccg 1561 gaccttctca tctttcaaat ggtcatcatc tgccttatgt gtgtgcacag cttggagaga 1621 gcaggatcca agcagtacag tgcagccatt gccttcaccc tggccctctt ttcccacctc 1681 gtcaatcatg tcaacatacg gctgcaggct gagctggaag agggcgagaa tcccgtcccg 1741 gcattccaga gtgatggcac agatgaacca gagtccaagg aacctgtgga gaaagaggag 1801 gagccagatc ctgagcctcc tcctgtaaca ccccaagtgg gtgagggcag aaagagccgt 1861 aagttctctc gcctctcctg tctccgccgt cgccgccacc cacccaaagt tggtgatgac 1921 agtgacctga gtgaaggctt tgaatcggac tcaagccatg actcagcccg ggccagtgag 1981 ggctcagaca gtggctctga caagagtctt gaaggtgggg gaacggcctt tgatgctgaa 2041 acagactcgg aaatgaatag ccaggagtcc cgatcagact tggaagatat ggaggaagag 2101 gaggggacac ggtcaccaac cctggagccc cctcggggca gatcagaggc tcccgattcc 2161 ctcaatggcc cactgggccc cagtgaggct agcattgcca gcaatctaca agccatgtcc 2221 acccagatgt tccagactaa gcgctgcttc cgactggccc ccacctttag caacctgctc 2281 ctccagccca ccaccaaccc tcatacctcg gccagccaca ggccttgcgt caatggggat 2341 gtagacaagc cttcagagcc agcctctgag gagggctctg agtcggaggg gagtgagtcc 2401 agtggacgct cctgtcggaa tgagcgcagc atccaggaga agcttcaggt cctgatggcc 2461 gaaggtctgc ttcctgctgt gaaagtcttc ctggactggc ttcggaccaa ccccgacctc 2521 atcatcgtgt gtgcgcagag ctctcaaagt ctgtggaacc gcctgtctgt gttgctgaat 2581 ctgttgcctg ctgctggtga actccaggag tctggcctgg ccttgtgtcc tgaggtccaa 2641 gatcttcttg aaggttgtga actgcctgac ctcccctcta gccttctgct cccagaggac 2701 atggctcttc gtaacctgcc cccgctccga gctgcccaca gacgctttaa ctttgacacg 2761 gatcggcccc tgctcagcac cttagaggag tcagtggtgc gcatctgctg catccgcagc 2821 tttggtcatt tcatcgcccg cctgcaaggc agcatcctgc agttcaaccc agaggttggc 2881 atcttcgtca gcattgccca gtctgagcag gagagcctgc tgcagcaggc ccaggcacag 2941 ttccgaatgg cacaggagga agctcgtcgg aacaggctca tgagagacat ggctcagcta 3001 cgacttcagc tcgaagtgtc tcagctggag ggcagcctgc agcagcccaa ggcccagtca 3061 gccatgtctc cctacctcgt ccctgacacc caggccctct gccaccatct ccctgtcatc 3121 cgccaactgg ccaccagtgg ccgcttcatt gtcatcatcc caaggacagt gatcgatggc 3181 ctggatttgc tgaagaagga acacccaggg gcccgggatg ggattcggta cctggaggca 3241 gagtttaaaa aaggaaacag gtacattcgc tgccagaaag aggtgggaaa gagctttgag 3301 cggcataagc tgaagaggca ggatgcagat gcctggactc tctataagat cctagacagc 3361 tgcaaacagc tgactctggc ccagggggca ggtgaggagg atccgagtgg catggtgacc 3421 atcatcacag gccttccact ggacaacccc agcgtgcttt caggccccat gcaggcagcc 3481 ctgcaggccg ctgcccacgc cagtgtggac atcaagaatg ttctggactt ctacaagcag 3541 tggaaggaaa ttggtCTCGA GTACCCATAC GACGTACCTG ATTATGCAAC TTATCCTTAT 3601 GATGTACCTG ATTATGCTAG CTATCCTTAT GATGTCCCAG ACTACGCATA GTGAGGGCCC 3661 GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA 3721 ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA 3781 TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT 3841 ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG 3901 CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT 3961 TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG 4021 GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC 4081 CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC 4141 CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC 4201 TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC 4261 CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC 4321 TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC 4381 GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC 4441 AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG 4501 GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT 4561 CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 4621 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 4681 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT 4741 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG 4801 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 4861 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT 4921 ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 4981 TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA 5041 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC 5101 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 5161 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG 5221 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC 5281 CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT 5341 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG 5401 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT 5461 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT 5521 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC 5581 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC 5641 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA 5701 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT 5761 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA 5821 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA 5881 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC 5941 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT 6001 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA 6061 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG 6121 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT 6181 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA ATGACCGACC 6241 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 6301 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 6361 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 6421 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 6481 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT 6541 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 6601 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 6661 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 6721 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 6781 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 6841 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 6901 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 6961 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 7021 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 7081 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 7141 CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 7201 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 7261 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 7321 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 7381 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 7441 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 7501 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 7561 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 7621 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 7681 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 7741 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 7801 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 7861 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 7921 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 7981 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 8041 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 8101 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 8161 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 8221 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 8281 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 8341 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 8401 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 8461 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 8521 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 8581 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 8641 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 8701 CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTCGACTC TCAGTACAAT 8761 CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC 8821 TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT 8881 GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.