pLV3-CMV-mCherry-H2BC1-3×FLAG-

pLV3-CMV-mCherry-H2BC1-3×FLAG-Puro plasmid

PVT96596

pLV3-CMV-mCherry-H2BC1-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-mCherry-H2BC1(human)-3×FLAG-Puro
Alias: NM_170610.3;bA317E16.3; H2BFU; HIST1H2BA; hTSH2B; STBP;TH2B; TSH2B; TSH2B.1
Gene lenght: 381bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: MCHERRY-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-mCherry-H2BC1-3×FLAG-Puro plasmid Description

pLV3-CMV-mCherry-H2BC1-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-mCherry-H2BC1-3×FLAG-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA 2341 GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA 2401 GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA 2461 GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA 2521 GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG 2581 CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA 2641 CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC 2701 CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT 2761 GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG 2821 CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC 2881 CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT 2941 CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA 3001 CAAGgGaGGT GGAGGTAGTG GAGGAGGCGG AtcAAGCTTG atgccggagg tgtcatctaa 3061 aggtgctacc atttccaaga agggctttaa gaaagctgtc gttaagaccc agaaaaagga 3121 aggcaaaaag cgcaagagga cccgtaagga gagttattct atttacatct acaaagtgct 3181 aaagcaggtc catccggaca ctggcatctc ttcgaaagct atgagcatta tgaattcctt 3241 cgtcactgat atctttgagc gtatagcgag cgaggcatca cgtttggctc actacagcaa 3301 gcgctccacc atttcttcca gagagattca gacagcagtg cgcttgctac tgccgggaga 3361 gctggctaaa catgctgtgt ctgagggcac caaggctgtc actaagtaca ccagctccaa 3421 gGGATCCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 3481 TGACGATGAC AAGTAgAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA 3541 CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG GGTGCCTAGA 3601 GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG 3661 AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG 3721 GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT TCACGCGCCC 3781 GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG CCTCCCGCCT 3841 GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG TCGAGACCGG 3901 GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC ACGCTTTGCC 3961 TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC CGTTACAGAT 4021 CCAAGCTGTG ACCGGCGCCT ACGCTAGATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC 4081 ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC 4141 GCCACGCGCC ACACCGTCGA CCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA 4201 CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC 4261 GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC 4321 GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC 4381 CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG 4441 CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC 4501 GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC 4561 GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG 4621 TGCATGACCC GCAAGCCCGG TGCCTGAgtc gacaatcaac ctctggatta caaaatttgt 4681 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 4741 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 4801 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 4861 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 4921 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 4981 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5041 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc 5101 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5161 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 5221 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 5281 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 5341 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 5401 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 5461 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 5521 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 5581 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 5641 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5701 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 5761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 5881 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 5941 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 6001 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 6061 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 6121 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 6181 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 6241 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 6301 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 6361 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6421 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6481 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6541 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6601 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6661 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6721 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 6781 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 6901 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 6961 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 7021 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 7081 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 7141 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 7201 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 7261 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 7321 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 7381 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 7441 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 7501 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 7561 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 7621 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 7681 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 7741 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 7801 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7861 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 7921 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 7981 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 8041 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 8101 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 8161 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 8221 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 8281 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 8341 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 8401 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 8461 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 8521 cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.