pLV2-TRE3GS-CDKN1A-EGFP-TetOne

pLV2-TRE3GS-CDKN1A-EGFP-TetOne-IRES-Puro plasmid

PVT87331

pLV2-TRE3GS-CDKN1A-EGFP-TetOne-IRES-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-CDKN1A(human)-EGFP-TetOne-IRES-Puro
Alias: NM_000389.5;CAP20; CDKN1; CIP1; MDA-6; P21; p21CIP1;SDI1; WAF1
Gene lenght: 492bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV2-TRE3GS-MCS-EGFP-TetOne-IRES-Puro
Forward primer:
Reverse primer: pEGFP-N-3'
Copy number: High
Induction:

pLV2-TRE3GS-CDKN1A-EGFP-TetOne-IRES-Puro plasmid Description

pLV2-TRE3GS-CDKN1A-EGFP-TetOne-IRES-Puro This is a plasmid suitable for the overexpression in mammalian cells. The plasmid carries the TRE3GS promoter, which can initiate the expression of the human CDKN1A gene. The plasmid also carries the EGFP green fluorescent protein at the C-terminus of the CDKN1A gene, facilitating the visual observation of the location and level of the target gene expression. The TRE3GS promoter

pLV2-TRE3GS-CDKN1A-EGFP-TetOne-IRES-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg tCCATAGAGC CCACCGCATC CCCAGCATGC CTGCTATTGT CTTCCCAATC 4141 CTCCCCCTTG CTGTCCTGCC CCACCCCACC CCCCAGAATA GAATGACACC TACTCAGACA 4201 ATGCGATGCA ATTTCCTCAT TTTATTAGGA AAGGACAGTG GGAGTGGCAC CTTCCAGGGT 4261 CAAGGAAGGC ACGGGGGAGG GGCAAACAAC AGATGGCTGG CAACTAGAAG GCACAGTCGA 4321 GGCTGATCAG CGGGTTTAAA ctaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt 4381 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg 4441 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact 4501 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg 4561 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt 4621 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac 4681 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc 4741 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg 4801 gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg 4861 aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc 4921 gtgggcttgt actcggtcat ggaaggtcgt ctccttgtgg gttgtggcaa gcttatcatc 4981 gtgtttttca aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc 5041 tcgactaaac acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg 5101 gtaccttctg ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc 5161 tttccacaac tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat 5221 cttatacacg tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc 5281 tgcaaagggt cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct 5341 tcacgacatt caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc 5401 tcgtcaagaa gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg 5461 tggaaaataa catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa 5521 cgttaggggg gggggaggga gaggggcgat tcgcgcgttt acccggggag catgtcaagg 5581 tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg 5641 gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac 5701 tttagctgtt tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg 5761 gctccctgcc ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg 5821 gtaggtgtct ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc 5881 agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc 5941 tgacacagga acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg 6001 ccgagatgca ctttagcccc gtcgcgatgt gagaggagag cacagcggta tgacttggcg 6061 ttgttccgca gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc 6121 ctgtccagca tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag 6181 tacagggtag gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg 6241 ataccgactc cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta 6301 gacatggtaa ttcgatgatc cccctgggga gagaggtcgg tgattcggtc aacgagggag 6361 ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac 6421 agggcccaca ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc 6481 tcggcgcgcc ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc 6541 attgctccct ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt 6601 cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc 6661 gtcgccgggg ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg 6721 aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag 6781 ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa 6841 ttatcacctc gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta 6901 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga 6961 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag 7021 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta 7081 tcagtgatag agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc 7141 tgattttttg agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc 7201 acttcctacc ctcgtaaaga attcGCCACC atgtcagaac cggctgggga tgtccgtcag 7261 aacccatgcg gcagcaaggc ctgccgccgc ctcttcggcc cagtggacag cgagcagctg 7321 agccgcgact gtgatgcgct aatggcgggc tgcatccagg aggcccgtga gcgatggaac 7381 ttcgactttg tcaccgagac accactggag ggtgacttcg cctgggagcg tgtgcggggc 7441 cttggcctgc ccaagctcta ccttcccacg gggccccggc gaggccggga tgagttggga 7501 ggaggcaggc ggcctggcac ctcacctgct ctgctgcagg ggacagcaga ggaagaccat 7561 gtggacctgt cactgtcttg tacccttgtg cctcgctcag gggagcaggc tgaagggtcc 7621 ccaggtggac ctggagactc tcagggtcga aaacggcggc agaccagcat gacagatttc 7681 taccactcca aacgccggct gatcttctcc aagaggaagc ccggatccgc caccatggtg 7741 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 7801 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 7861 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 7921 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 7981 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 8041 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 8101 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 8161 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 8221 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 8281 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 8341 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 8401 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtagagacca 8461 cctcccctgc gagctaagct ggacagccaa tgacgggtaa gagagtgaca tttttcacta 8521 acctaagaca ggagggccgt cagagctact gcctaatcca aagacgggta aaagtgataa 8581 aaatgtatca ctccaaccta agacaggcgc agcttccgag ggatttgaga tccagacatg 8641 ataagataca ttgatgagtt tggacaaacc aaaactagaa tgcagtgaaa aaaatgcctt 8701 atttgtgaaa tttgtgatgc tattgcctta tttgtaacca ttataagctg caataaacaa 8761 gttaagtcga taaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt 8821 tgtaatttgt ttttgtagtt ctttagtttg tatgtctgtt gctattatgt ctactattct 8881 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaggcgatac cgtcgagatc 8941 cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt 9001 cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc 9061 cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc 9121 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat 9181 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt 9241 tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc 9301 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat 9361 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct 9421 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc 9481 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct 9541 gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc 9601 ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc 9661 ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa 9721 tttttactac tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc 9781 caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc 9841 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca 9901 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga 9961 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt 10021 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt 10081 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat 10141 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt 10201 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg 10261 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta 10321 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac 10381 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg 10441 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta 10501 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc 10561 caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag ctcgatatca 10621 gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg ctaggcggct 10681 gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca ggctcacagg 10741 gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg gctcaactgg 10801 tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc ctggtgtgta 10861 gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg 10921 tcttctttgg gagtgaatta gcccttcca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.