pLV2-TRE3GS-MCS-IRES-tdtomato-

pLV2-TRE3GS-MCS-IRES-tdtomato-TetOne-Puro plasmid

PVT91756

pLV2-TRE3GS-MCS-IRES-tdtomato-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-MCS-IRES-tdtomato-TetOne-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-MCS-IRES-tdtomato-TetOne-Puro plasmid Description

pLV2-TRE3GS-MCS-IRES-tdtomato-TetOne-Puro is a plasmid suitable for protein expression in mammalian cells, tetracycline-inducible, and can also be used for lentivirus packaging.

pLV2-TRE3GS-MCS-IRES-tdtomato-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcggatc cgggcccgcg 6721 gccgccccgg ccggcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg 6781 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc 6841 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc 6901 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa 6961 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct 7021 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca 7081 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctcacctca 7141 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat 7201 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc 7261 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataaGCC ACCatggtga 7321 gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca 7381 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 7441 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt 7501 ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt 7561 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 7621 gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg 7681 tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg 7741 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc 7801 accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca 7861 tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca 7921 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc 7981 acctgttcct ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct 8041 ccgaggacaa caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg 8101 gctccatgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 8161 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca 8221 tcctgtcccc ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc 8281 ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 8341 aggacggcgg tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct 8401 acaaggtgaa gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga 8461 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg 8521 agatccacca ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca 8581 tctacatggc caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg 8641 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc 8701 gccaccacct gttcctgtac ggcatggacg agctgtacaa gtagggatcc agaccacctc 8761 ccctgcgagc taagctggac agccaatgac gggtaagaga gtgacatttt tcactaacct 8821 aagacaggag ggccgtcaga gctactgcct aatccaaaga cgggtaaaag tgataaaaat 8881 gtatcactcc aacctaagac aggcgcagct tccgagggat ttgagatcca gacatgataa 8941 gatacattga tgagtttgga caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt 9001 gtgaaatttg tgatgctatt gccttatttg taaccattat aagctgcaat aaacaagtta 9061 agtcgataaa ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta 9121 atttgttttt gtagttcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc 9181 cctgcactgt accccccaat cccccctttt cttttaaagg cgataccgtc gagatccgtt 9241 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg 9301 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg 9361 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc 9421 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca 9481 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg 9541 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc 9601 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca 9661 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg 9721 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac 9781 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg 9841 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat 9901 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt 9961 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt 10021 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg 10081 tctgaagatc agcggccggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc 10141 tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag 10201 agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt 10261 tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact 10321 gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt 10381 ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg 10441 acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc 10501 accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga 10561 gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga 10621 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac 10681 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc 10741 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg 10801 caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg 10861 ccacgcctcc ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag 10921 ttcttgaagt actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca 10981 aacctccact ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt 11041 aacaagctgg tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact 11101 agcttgtagc accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc 11161 tgctaatcag ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt 11221 ctttgggagt gaattagccc ttcca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.