pCMV-ZRANB1-6×His-Neo plasmid

pCMV-ZRANB1-6×His-Neo plasmid

PVT87224

pCMV-ZRANB1-6×His-Neo plasmid Information

Plasmid name: pCMV-ZRANB1(human)-6×His-Neo
Alias: NM_017580.3;TRABID
Gene lenght: 2124bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCDNA3.1-MCS-6×His
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ZRANB1-6×His-Neo plasmid Description

pCMV-ZRANB1-6×His-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the transcription of the downstream human ZRANB1 gene, promoting the C-terminal fusion expression of the ZRANB1 gene with a 6×His tag, which facilitates experiments such as protein immunoprecipitation and co-immunoprecipitation. The plasmid carries a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-ZRANB1-6×His-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCATG TCAGAACGTG GAATTAAGTG GGCTTGTGAA TATTGTACGT ATGAAAACTG 961 GCCATCTGCA ATCAAGTGTA CTATGTGTCG TGCCCAAAGA CCTAGTGGAA CAATTATTAC 1021 AGAAGATCCA TTTAAAAGTG GTTCAAGTGA TGTTGGTAGA GATTGGGATC CTTCCAGCAC 1081 CGAAGGAGGA AGTAGTCCTT TGATATGTCC AGACTCTAGT GCAAGACCAA GGGTGAAATC 1141 TTCGTATAGC ATGGAAAATG CAAATAAGTG GTCATGCCAC ATGTGTACAT ATTTGAACTG 1201 GCCAAGAGCA ATCAGATGTA CCCAGTGCTT ATCCCAACGT AGGACCAGGA GTCCTACAGA 1261 ATCTCCTCAG TCCTCAGGAT CTGGCTCAAG ACCAGTTGCT TTTTCTGTTG ATCCTTGTGA 1321 GGAATACAAT GATAGAAATA AACTGAACAC TAGGACACAG CACTGGACTT GCTCTGTTTG 1381 CACATATGAA AACTGGGCCA AGGCTAAAAG ATGTGTTGTT TGTGATCATC CCAGACCTAA 1441 TAACATTGAA GCAATAGAAT TGGCAGAGAC TGAAGAGGCT TCTTCAATAA TAAATGAGCA 1501 AGACAGAGCT CGATGGAGGG GAAGTTGCAG TAGTGGTAAT AGCCAAAGGA GATCACCTCC 1561 TGCTACGAAG CGGGACTCTG AAGTGAAAAT GGATTTTCAG AGGATTGAAT TGGCTGGTGC 1621 TGTGGGAAGC AAGGAGGAAC TTGAAGTAGA CTTTAAAAAA CTAAAGCAAA TTAAAAACAG 1681 GATGAAAAAG ACTGATTGGC TCTTCCTCAA TGCTTGTGTG GGGGTTGTAG AAGGTGATTT 1741 AGCTGCCATA GAAGCATACA AGTCATCAGG AGGAGACATT GCACGTCAGC TCACCGCAGA 1801 TGAAGTACGC TTGCTGAATC GTCCTTCTGC CTTTGATGTT GGCTATACTC TTGTACACTT 1861 GGCTATACGT TTTCAGAGGC AGGATATGCT AGCAATATTG CTTACAGAGG TGTCTCAACA 1921 AGCAGCAAAG TGTATTCCAG CAATGGTGTG TCCTGAACTG ACAGAACAAA TCCGGAGAGA 1981 GATAGCTGCC TCTCTTCATC AGAGAAAGGG GGATTTTGCT TGCTATTTTC TGACTGACCT 2041 TGTAACATTT ACATTGCCAG CAGATATTGA AGATTTGCCC CCAACAGTCC AAGAAAAATT 2101 ATTTGATGAG GTGCTTGATA GAGACGTTCA AAAAGAATTA GAAGAAGAAT CTCCAATTAT 2161 TAACTGGTCC TTGGAATTGG CTACACGTTT GGACAGTCGA CTGTATGCAC TTTGGAACCG 2221 GACTGCAGGA GACTGCCTAC TTGATTCAGT TCTACAAGCT ACCTGGGGCA TCTATGACAA 2281 GGACTCAGTG CTTCGGAAAG CCCTGCATGA CAGCCTGCAT GACTGTTCAC ATTGGTTTTA 2341 CACACGCTGG AAAGATTGGG AATCATGGTA TTCTCAGAGC TTTGGTTTAC ATTTTTCCTT 2401 GAGAGAAGAA CAGTGGCAAG AAGACTGGGC ATTTATACTC TCTCTTGCTA GTCAGCCTGG 2461 AGCAAGCTTG GAGCAGACGC ACATTTTTGT ACTGGCACAT ATTCTTAGAC GACCAATTAT 2521 AGTTTATGGA GTAAAATATT ACAAGAGTTT CCGGGGAGAA ACTTTAGGAT ATACTCGGTT 2581 TCAAGGTGTT TATCTGCCTT TGTTGTGGGA ACAGAGTTTT TGTTGGAAAA GTCCGATTGC 2641 TCTGGGTTAT ACGAGGGGCC ACTTCTCTGC TTTGGTTGCC ATGGAAAATG ATGGCTATGG 2701 CAACCGAGGT GCTGGTGCTA ATCTCAATAC CGATGATGAT GTCACCATCA CATTTTTGCC 2761 TCTGGTTGAC AGTGAAAGGA AGCTACTCCA TGTGCACTTC CTTTCTGCTC AGGAGCTAGG 2821 TAATGAGGAA CAGCAAGAAA AACTGCTCAG GGAGTGGCTG GACTGCTGTG TGACGGAGGG 2881 GGGAGTTCTG GTTGCCATGC AGAAGAGTTC TCGGCGGCGA AATCACCCCC TGGTCACTCA 2941 GATGGTAGAA AAATGGCTTG ACCGCTACCG ACAGATCCGG CCGTGTACAT CCCTGTCTGA 3001 TGGAGAGGAA GATGAGGATG ATGAAGATGA AGCGGCCGCA CATCACCATC ATCACCATTG 3061 ACTCGAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG 3121 CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC 3181 CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC 3241 TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG 3301 GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC 3361 TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 3421 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 3481 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT 3541 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 3601 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 3661 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 3721 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 3781 TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG 3841 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 3901 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 3961 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT 4021 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC 4081 GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT 4141 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA 4201 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 4261 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 4321 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC 4381 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 4441 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA 4501 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 4561 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 4621 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT 4681 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 4741 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC 4801 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 4861 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 4921 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC 4981 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 5041 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 5101 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 5161 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 5221 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 5281 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 5341 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 5401 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 5461 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 5521 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 5581 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 5641 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 5701 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 5761 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 5821 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 5881 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 5941 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 6001 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 6061 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 6121 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 6181 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 6241 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT 6301 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 6361 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 6421 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 6481 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 6541 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 6601 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 6661 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 6721 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 6781 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 6841 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 6901 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 6961 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 7021 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 7081 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 7141 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 7201 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 7261 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 7321 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 7381 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 7441 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 7501 ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.