pCMV-VPS8-EGFP-Neo plasmid

pCMV-VPS8-EGFP-Neo plasmid

PVT87178

pCMV-VPS8-EGFP-Neo plasmid Information

Plasmid name: pCMV-VPS8(human)-EGFP-Neo
Alias: NM_001009921.3;KIAA0804
Gene lenght: 4284bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-EGFP-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-VPS8-EGFP-Neo plasmid Description

pCMV-VPS8-EGFP-Neo This is a plasmid suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the downstream human VPS8 gene. The plasmid carries EGFP green fluorescent protein at the C-terminus of the VPS8 gene, facilitating the visual observation of the location and level of the target gene expression. The plasmid contains a Neo/G418 resistance gene for selecting host cells that express the target gene.

pCMV-VPS8-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc gccaccatgg aaaatgaacc agaccatgaa aatgtggaac 961 agagcctctg tgccaagacg agcgaagaag agctgaataa gtctttcaat ctagaagctt 1021 cactttcaaa attctcttac atagatatgg acaaggaact ggagttcaaa aatgatctga 1081 ttgatgacaa ggagtttgat attcctcaag ttgatactcc tccaacactg gaaagcatac 1141 taaatgagac tgatgatgaa gatgagtctt ttattcttga ggatcctaca ttgttaaaca 1201 ttgatactat tgattctcac tcctatgata cttcatctgt ggcaagctca gatagtggtg 1261 acaggaccaa cttaaaaagg aagaagaaat tacctgattc tttttcactt catggatcag 1321 ttatgcgcca ttcacttttg aagggaattt ctgcccagat agtgtctgca gctgacaaag 1381 tagatgctgg cttgcctaca gcaattgcag tatccagtct gatagcagtg ggtacatctc 1441 atggattggc tttaatattt ggaaaagatc agaatcaagc tttgcgactc tgtctgggta 1501 gcactagtgt tggaggtcag tatggcgcta tctctgccct cagtatcaac aatgattgct 1561 caagacttct ttgtggcttt gctaaaggac agatcaccat gtgggatttg gccagtggaa 1621 aacttctaag atcaataaca gatgctcatc ctccaggaac agcaatattg catatcaagt 1681 ttacagatga tccaactctt gcaatttgca acgacagcgg aggctctgtt tttgaattga 1741 catttaagag agtgatggga gtgagaacct gtgaatctag atgtctgttc agtggttcca 1801 agggtgaagt ctgctgtatt gagcctctgc attctaagcc tgagttgaaa gatcatccca 1861 tcacacagtt ttcattattg gccatggcat ccttgacaaa aatactggtc attggattga 1921 aaccatcctt gaaagtatgg atgacttttc cctatggccg gatggatcct tccagtgtgc 1981 cactgctggc ctggcacttt gtagcagtac aaaattacgt gaatcccatg cttgccttct 2041 gcagaggaga tgttgttcat tttctattgg taaagagaga tgaatctgga gcaatacatg 2101 ttactaagca aaagcatctt cacctatact atgacctcat caactttacc tggataaatt 2161 cacgcacagt tgtgctctta gacagcgtag agaagttgca tgtgattgat cggcaaacac 2221 aagaggaatt ggagacagtg gagatctcag aagttcagct ggtctacaat agcagccatt 2281 tcaaatcact agccactgga ggaaatgtta gccaggcact ggctttggtt ggagagaagg 2341 cttgttatca atccatcagt agctatggtg gtcagatctt ttatttgggg acaaaatctg 2401 tttatgtgat gatgctgagg agctggagag agagagtgga tcatctcctg aaacaagatt 2461 gtcttacaga agcgttggct cttgcgtggt ctttccatga aggaaaagca aaagcagtag 2521 tgggattatc aggggatgcc agtaagcgaa aggctattgt tgcagaccgg atggtagaaa 2581 tcctattcca ttatgcagat cgagctctga aaaagtgccc agaccaagga aaaatccaag 2641 tgatggagca gcattttcag gatatggtgc ctgtcatagt tgattactgc cttctgctgc 2701 agcgaaagga tcttttattt agtcagatgt atgataaatt aagtgagaat tcagtggcca 2761 aaggagtatt tttggagtgc cttgagccat atattttaag tgataaattg gtgggaatca 2821 caccccaagt aatgaaagac ttgattgttc atttccaaga taaaaaatta atggaaaatg 2881 tggaagcgct cattgtacat atggatatca ccagcctaga tattcagcag gtagttctca 2941 tgtgttggga aaatcgttta tatgatgcta tgatctatgt ctacaacaga ggcatgaatg 3001 aatttattag tccaatggag aaacttttca gagtcattgc tcctcctctg aatgcaggaa 3061 aaacactaac agatgaacaa gttgttatgg gcaataagct ccttgtatat attagctgtt 3121 gtctagcagg tcgtgcctat ccccttggtg acatccctga agatctggtt cccttggtta 3181 aaaaccaggt ttttgaattt ctaattcgcc tgcattcagc agaggcttct cctgaggaag 3241 aaatctatcc ttacattcgg actttgctac attttgacac aagagaattt ctaaatgtat 3301 tggcactgac ttttgaagat tttaaaaatg acaagcaagc tgtggaatat caacagcgaa 3361 ttgtggatat tttgttgaaa gttatggtgg agaattcaga ctttaccccc tcacaagtag 3421 gatgtctctt taccttcctt gctcggcagc ttgcaaagcc tgacaacacc ttgtttgtaa 3481 acagaacact ttttgatcag gtccttgaat tcctttgtag tcctgacgat gactcccgac 3541 actctgaaag acagcaggtc cttttagaat tgctgcaggc tggaggcata gttcaatttg 3601 aagagagtcg actcatccgg atggcagaaa aagctgagtt ctatcaaatt tgtgaattta 3661 tgtatgaaag agaacaccaa tatgataaaa ttattgattg ctacttacgt gaccctctgc 3721 gagaggaaga agtctttaat tacattcaca atatcttatc cattcccgga cacagtgcag 3781 aggagaagca gtctgtatgg cagaaagcaa tggatcatat tgaggaactc gtgtccctga 3841 agccttgtaa agctgcggag ctggttgcca cccacttttc tggacatatt gaaacggtca 3901 ttaaaaaact tcagaaccag gttttgcttt tcaaattttt gaggagtctt cttgacccaa 3961 gggaaggtat tcatgtaaat caagaattac tgcaaatatc tccttgtatc acagagcagt 4021 tcattgagct gttgtgtcag ttcaacccaa cccaagttat agagactctg caagtccttg 4081 agtgctaccg tctggaagaa actattcaga ttactcagaa gtatcaactt catgaagtca 4141 ccgcttatct attggaaaag aaaggagata ttcatggtgc cttcctaata atgttagaga 4201 gactacaaag caaacttcaa gaggtaacac atcaaggtga aaataccaaa gaggatccct 4261 cattgaagga tgttgaagat actatggtgg agaccattgc tctttgccag agaaattcac 4321 ataatttgaa ccagcagcaa cgtgaggcac tttggtttcc gttattggag gcaatgatgg 4381 cccctcagaa gctgtccagt tcagccattc ctcatctaca ctctgaagct ctgaagtctt 4441 tgaccatgca agttttaaat agcatggcag catttattgc ccttccatca atcttgcaaa 4501 gaatcttaca ggatccagtt tatggaaaag gaaaacttgg agaaatccag ggacttatct 4561 tgggaatgtt agataccttt aactatgaac aaaccctgct ggaaacaaca accagccttc 4621 taaaccaaga tctccattgg tcattgtgta acctgagagc ttcggtcacc agaggactga 4681 atcccaaaca agattactgc tctatatgtt tgcagcagta caagagacgc caagaaatgg 4741 ctgatgaaat aattgtcttt agctgtggcc atttgtatca ctcattctgc ctacaaaaca 4801 aagaatgcac tgtggaattt gagggccaaa caagatggac atgctacaaa tgcagttcaa 4861 gtaacaaagt aggaaaactc agtgaaaatt catctgaaat taaaaaggga aggataaccc 4921 catcacaggt aaaaatgtct ccatcgtatc atcagtccaa aggggatccc actgctaaaa 4981 agggaacctc agaacctgtt ctggatccac agcaaatcca agcatttgat cagctttgcc 5041 gtctctaccg aggaagctcc aggctggctc tcctcacgga actctcccag aatcgcagca 5101 gcgagagcta taggccattc agtggctcgc agagtgctcc tgctttcaac agcatcttcc 5161 agaatgagaa cttccagctg cagctcattc ctccacctgt gactgaggat ctcgagggcg 5221 gtggaggaag cggcggaggt ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg 5281 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 5341 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 5401 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 5461 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 5521 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 5581 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 5641 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 5701 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 5761 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 5821 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 5881 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 5941 tcggcatgga cgagctgtac aagtagtcta gagggcccgt ttaaacccgc tgatcagcct 6001 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 6061 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 6121 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 6181 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg 6241 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg 6301 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 6361 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 6421 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 6481 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 6541 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 6601 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 6661 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg 6721 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 6781 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 6841 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc 6901 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat 6961 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt 7021 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc 7081 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 7141 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 7201 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 7261 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 7321 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 7381 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 7441 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 7501 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 7561 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 7621 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 7681 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 7741 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 7801 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 7861 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg 7921 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc 7981 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc 8041 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat 8101 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 8161 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg 8221 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 8281 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 8341 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 8401 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 8461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 8521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 8581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 8641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 8701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 8761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 8821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 8881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 8941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 9001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 9061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 9121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 9181 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 9241 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 9301 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 9361 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 9421 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 9481 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 9541 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 9601 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 9661 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 9721 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 9781 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 9841 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 9901 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 9961 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 10021 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 10081 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 10141 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 10201 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 10261 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 10321 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 10381 ccccgaaaag tgccacctga cgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.