pT7-6×His-TRIM28-6×His plasmid

pT7-6×His-TRIM28-6×His plasmid

PVT86316

pT7-6×His-TRIM28-6×His plasmid Information

Plasmid name: pT7-6×His-TRIM28(human)-6×His
Alias: NM_005762.3;KAP1; PPP1R157; RNF96; TF1B; TIF1B;TIF1beta;
Gene lenght: 2505bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling:
Promoter: T7
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pET-28a
Forward primer: T7
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pT7-6×His-TRIM28-6×His plasmid Description

pT7-6×His-TRIM28-6×His This is a prokaryotic inducible low-copy expression plasmid. The plasmid contains a T7-LacO promoter system that, under the induction of IPTG or lactose and its analogs, initiates the expression of the human TRIM28 gene. The N-terminus of the TRIM28 gene in the plasmid carries His, Thrombin, and T7 protein tags, while the C-terminus has a His tag, which facilitates subsequent studies on the detection, purification, and localization of the expressed protein.

pT7-6×His-TRIM28-6×His plasmid Sequence

1 TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG 61 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC 121 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG 181 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC 241 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT 301 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC 361 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA 421 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT 481 TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA 541 TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT 601 TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA 661 ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC 721 GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA 781 AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC 841 AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC 901 CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC 961 AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT 1021 TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG 1081 TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA 1141 TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC 1201 CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG 1261 TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA 1321 TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC 1381 CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA 1441 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 1501 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 1561 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 1621 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 1681 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 1741 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 1801 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 1861 ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 1921 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 1981 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 2041 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 2101 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 2161 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 2221 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG 2281 TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA 2341 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG 2401 GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT 2461 GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG 2521 GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC 2581 GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG 2641 AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT 2701 GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA 2761 ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG 2821 TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG 2881 TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC 2941 TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA 3001 CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA 3061 GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC 3121 CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC 3181 CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA 3241 GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC 3301 GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC 3361 GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA 3421 CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA 3481 ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 3541 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 3601 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA 3661 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA 3721 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT 3781 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG 3841 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA 3901 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA 3961 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG 4021 AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT 4081 GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT 4141 GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG 4201 CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT 4261 TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC 4321 TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA 4381 GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG 4441 CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT 4501 TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG 4561 CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT 4621 CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA 4681 TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG 4741 CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC 4801 CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG 4861 CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG 4921 GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA 4981 AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA 5041 TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC 5101 AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGCTAGCA TGACTGGTGG ACAGCAAATG 5161 GGTCGCGGAT CCATGGCGGC CTCCGCGGCG GCAGCCTCGG CAGCAGCGGC CTCGGCCGCC 5221 TCTGGCAGCC CGGGCCCGGG CGAGGGCTCC GCTGGCGGCG AAAAGCGCTC CACCGCCCCT 5281 TCGGCCGCAG CCTCGGCCTC TGCCTCAGCC GCGGCGTCGT CGCCCGCGGG GGGCGGCGCC 5341 GAGGCGCTGG AGCTGCTGGA GCACTGCGGC GTGTGCAGAG AGCGCCTGCG ACCCGAGAGG 5401 GAGCCCCGCC TGCTGCCCTG TTTGCACTCG GCCTGTAGTG CCTGCTTAGG GCCCGCGGCC 5461 CCCGCCGCCG CCAACAGCTC GGGGGACGGC GGGGCGGCGG GCGACGGCAC CGTGGTGGAC 5521 TGTCCCGTGT GCAAGCAACA GTGCTTCTCC AAAGACATCG TGGAGAATTA TTTCATGCGT 5581 GATAGTGGCA GCAAGGCTGC CACCGACGCC CAGGATGCGA ACCAGTGCTG CACTAGCTGT 5641 GAGGATAATG CCCCAGCCAC CAGCTACTGT GTGGAGTGCT CGGAGCCTCT GTGTGAGACC 5701 TGTGTAGAGG CGCACCAGCG GGTGAAGTAC ACCAAGGACC ATACTGTGCG CTCTACTGGG 5761 CCAGCCAAGT CTCGGGATGG TGAACGTACT GTCTATTGCA ACGTACACAA GCATGAACCC 5821 CTTGTGCTGT TTTGTGAGAG CTGTGATACT CTCACCTGCC GAGACTGCCA GCTCAATGCC 5881 CACAAGGACC ACCAGTACCA GTTCTTAGAG GATGCAGTGA GGAACCAGCG CAAGCTCCTG 5941 GCCTCACTGG TGAAGCGCCT TGGGGACAAA CATGCAACAT TGCAGAAGAG CACCAAGGAG 6001 GTTCGCAGCT CAATCCGCCA GGTGTCTGAC GTACAGAAGC GTGTGCAAGT GGATGTCAAG 6061 ATGGCCATCC TGCAGATCAT GAAGGAGCTG AATAAGCGGG GCCGTGTGCT GGTCAATGAT 6121 GCCCAGAAGG TGACTGAGGG GCAGCAGGAG CGCCTGGAGC GGCAGCACTG GACCATGACC 6181 AAGATCCAGA AGCACCAGGA GCACATTCTG CGCTTTGCCT CTTGGGCTCT GGAGAGTGAC 6241 AACAACACAG CCCTTTTGCT TTCTAAGAAG TTGATCTACT TCCAGCTGCA CCGGGCCCTC 6301 AAGATGATTG TGGATCCCGT GGAGCCACAT GGCGAGATGA AGTTTCAGTG GGACCTCAAT 6361 GCCTGGACCA AGAGTGCCGA GGCCTTTGGC AAGATTGTGG CAGAGCGTCC TGGCACTAAC 6421 TCAACAGGCC CTGCACCCAT GGCCCCTCCA AGAGCCCCAG GGCCCCTGAG CAAGCAGGGC 6481 TCTGGCAGCA GCCAGCCCAT GGAGGTGCAG GAAGGCTATG GCTTTGGGTC AGGAGATGAT 6541 CCCTACTCAA GTGCAGAGCC CCATGTGTCA GGTGTGAAAC GGTCCCGCTC AGGTGAGGGC 6601 GAGGTGAGCG GCCTTATGCG CAAGGTGCCA CGAGTGAGCC TTGAACGCCT GGACCTGGAC 6661 CTCACAGCTG ACAGCCAGCC ACCCGTCTTC AAGGTCTTCC CAGGCAGTAC CACTGAGGAC 6721 TACAACCTTA TTGTTATTGA ACGTGGCGCT GCCGCTGCAG CTACCGGCCA GCCAGGGACT 6781 GCGCCTGCAG GAACCCCTGG TGCCCCACCC CTGGCTGGCA TGGCCATTGT CAAGGAGGAG 6841 GAGACGGAGG CTGCCATTGG AGCCCCTCCT ACTGCCACTG AGGGCCCTGA GACCAAACCT 6901 GTGCTTATGG CTCTTGCGGA GGGTCCTGGT GCTGAGGGTC CCCGCCTGGC CTCACCTAGT 6961 GGCAGCACCA GCTCAGGGCT GGAGGTGGTG GCTCCTGAGG GTACCTCAGC CCCAGGTGGT 7021 GGCCCGGGAA CCCTGGATGA CAGTGCCACC ATTTGCCGTG TCTGCCAGAA GCCAGGCGAT 7081 CTGGTTATGT GCAACCAGTG TGAGTTTTGT TTCCACCTGG ACTGTCACCT GCCGGCCCTG 7141 CAGGATGTAC CAGGGGAGGA GTGGAGCTGC TCACTCTGCC ATGTGCTCCC TGACCTGAAG 7201 GAGGAGGATG GCAGCCTCAG CCTGGATGGT GCAGACAGCA CTGGCGTGGT GGCCAAGCTC 7261 TCACCAGCCA ACCAGCGGAA ATGTGAGCGT GTACTGCTGG CCCTATTCTG TCACGAACCC 7321 TGCCGCCCCC TGCATCAGCT GGCTACCGAC TCCACCTTCT CCCTGGACCA GCCCGGTGGC 7381 ACCCTGGATC TGACCCTGAT CCGTGCCCGC CTCCAGGAGA AGTTGTCACC TCCCTACAGC 7441 TCCCCACAGG AGTTTGCCCA GGATGTGGGC CGCATGTTCA AGCAATTCAA CAAGTTAACT 7501 GAGGACAAGG CAGACGTGCA GTCCATCATC GGCCTGCAGC GCTTCTTCGA GACGCGCATG 7561 AACGAGGCCT TCGGTGACAC CAAGTTCTCT GCTGTGCTGG TGGAGCCCCC GCCGATGAGC 7621 CTGCCTGGTG CTGGCCTGAG TTCCCAGGAG CTGTCTGGTG GCCCTGGTGA TGGCCCCCTC 7681 GAGCACCACC ACCACCACCA CTGAGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG 7741 TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 7801 TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.