pCMV-3×FLAG-HECW2-Neo plasmid

pCMV-3×FLAG-HECW2-Neo plasmid

PVT86256

pCMV-3×FLAG-HECW2-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-HECW2(human)-Neo
Alias: NM_020760.4;NDHSAL; NEDL2;
Gene lenght: 4719bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-T7-3×FLAG-MCS-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-HECW2-Neo plasmid Description

pCMV-3×FLAG-HECW2-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the human HECW2 gene, promoting the N-terminal fusion expression of a 3×FLAG tag, which facilitates the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. The plasmid carries a Neo/G418 resistance gene, which can be used to select host cells expressing the target gene.

pCMV-3×FLAG-HECW2-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCCACCAT GGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 961 ATCGACTACA AGGATGACGA TGACAAGCTT ATGGCTAGTT CAGCCCGGGA GCACCTGCTT 1021 TTTGTGAGGC GTCGAAATCC CCAGATGCGG TACACATTGA GCCCAGAGAA CCTCCAGAGC 1081 CTTGCCGCCC AGAGCTCCAT GCCAGAGAAC ATGACCCTGC AGCGGGCCAA CAGCGACACC 1141 GACCTGGTGA CTTCTGAGAG CCGCTCCAGC TTAACTGCCA GCATGTACGA GTACACGCTG 1201 GGGCAAGCCC AGAACCTCAT TATCTTCTGG GACATTAAAG AGGAGGTGGA CCCCAGTGAT 1261 TGGATTGGAC TTTATCATAT AGATGAGAAT TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC 1321 AGGGGTGTGA CTGGAACACA AAAAGGGCAA ATTGTATGGA GAATTGAGCC TGGGCCCTAT 1381 TTCATGGAAC CGGAGATAAA AATCTGTTTT AAATATTACC ACGGCATTAG TGGAGCCCTG 1441 CGAGCCACGA CCCCCTGCAT CACCGTGAAG AACCCAGCTG TGATGATGGG GGCAGAAGGC 1501 ATGGAGGGAG GTGCTTCAGG AAACCTGCAT TCTCGAAAAC TTGTTAGCTT TACATTGTCA 1561 GATCTTAGGG CAGTTGGGCT AAAGAAAGGG ATGTTCTTCA ATCCTGACCC TTATCTTAAG 1621 ATGTCAATTC AGCCAGGAAA GAAGAGCAGT TTCCCCACCT GTGCCCACCA CGGGCAGGAG 1681 AGACGGTCTA CTATCATCAG TAACACCACC AATCCAATTT GGCACCGAGA GAAATATTCC 1741 TTTTTTGCAC TTCTTACTGA TGTCTTAGAA ATTGAAATTA AAGACAAATT TGCCAAGAGC 1801 CGTCCCATCA TCAAGCGTTT TCTGGGGAAA CTAACCATTC CAGTCCAGAG GCTGCTGGAG 1861 CGACAAGCCA TCGGTGATCA AATGCTCAGC TACAACCTTG GCAGAAGGCT CCCAGCTGAC 1921 CACGTGAGTG GGTACCTCCA GTTTAAAGTG GAGGTTACGT CTTCTGTTCA TGAAGATGCC 1981 TCTCCAGAAG CTGTTGGCAC AATACTTGGA GTCAATTCTG TGAATGGAGA CTTAGGTAGC 2041 CCTTCCGATG ACGAGGACAT GCCAGGGAGC CATCACGACA GCCAGGTGTG CTCTAATGGG 2101 CCAGTTTCTG AGGACAGTGC TGCCGATGGA ACCCCCAAGC ATTCATTCAG GACTAGCTCC 2161 ACTCTGGAAA TAGACACAGA GGAATTAACC TCTACGTCTT CAAGGACCTC ACCTCCCAGA 2221 GGACGTCAGG ATTCACTCAA TGATTACTTA GATGCTATCG AACACAATGG CCACTCCAGG 2281 CCGGGGACAG CGACCTGCTC TGAGAGGTCC ATGGGTGCCT CTCCGAAACT GAGGAGTAGT 2341 TTTCCCACTG ACACAAGACT CAATGCCATG CTTCATATTG ATTCAGATGA AGAAGATCAC 2401 GAGTTCCAGC AAGACCTGGG TTACCCATCT TCCTTGGAGG AGGAGGGAGG CCTGATCATG 2461 TTTAGCAGGG CATCCAGAGC TGATGATGGA AGCCTGACAT CTCAGACAAA GCTGGAGGAC 2521 AACCCTGTTG AGAATGAGGA AGCCTCCACA CACGAAGCTG CTTCCTTTGA GGATAAGCCA 2581 GAAAATCTTC CAGAGCTTGC AGAGAGCTCC TTACCTGCAG GCCCAGCCCC AGAGGAAGGT 2641 GAAGGCGGCC CAGAGCCTCA ACCCAGTGCT GACCAGGGCA GTGCTGAACT GTGTGGCTCT 2701 CAAGAGGTAG ATCAGCCCAC AAGTGGCGCA GACACAGGGA CTTCCGATGC ATCAGGAGGA 2761 AGCCGAAGAG CCGTCAGTGA GACCGAGTCT CTCGATCAGG GCTCTGAGCC TTCCCAGGTG 2821 TCCTCTGAAA CAGAACCCAG TGATCCTGCC AGGACAGAGA GTGTGAGCGA AGCCAGCACC 2881 AGGCCTGAGG GAGAGAGTGA CCTGGAATGC GCTGACAGCT CCTGCAATGA GAGTGTGACC 2941 ACGCAGCTGT CCTCTGTGGA CACACGGTGC TCCTCTCTTG AGAGCGCACG GTTTCCTGAA 3001 ACCCCAGCCT TTTCTTCTCA GGAGGAGGAA GACGGAGCCT GTGCAGCAGA GCCCACCAGC 3061 AGTGGCCCTG CCGAAGGGTC GCAGGAATCC GTGTGCACTG CTGGTTCTTT ACCTGTGGTA 3121 CAGGTGCCCA GTGGGGAGGA TGAAGGGCCA GGGGCAGAAT CGGCCACTGT ACCTGACCAG 3181 GAGGAGCTGG GGGAGGTCTG GCAGCGGAGG GGGAGCCTGG AGGGAGCTGC AGCTGCTGCC 3241 GAGAGCCCAC CGCAAGAAGA AGGCAGTGCT GGGGAGGCCC AAGGCACCTG TGAAGGGGCA 3301 ACTGCCCAGG AGGAGGGCGC TACTGGAGGT TCTCAAGCCA ACGGCCACCA GCCACTGCGA 3361 TCACTACCTT CAGTGCGCCA GGATGTTAGC CGGTACCAGA GGGTGGACGA GGCTCTCCCA 3421 CCAAACTGGG AGGCACGCAT TGACAGCCAC GGCAGGATCT TCTACGTGGA TCACGTAAAC 3481 AGAACCACGA CGTGGCAGCG ACCGACAGCT CCCCCAGCCC CGCAGGTGCT GCAGAGATCT 3541 AACTCCATAC AGCAAATGGA GCAGCTGAAC CGGCGATATC AGAGTATCCG CAGAACCATG 3601 ACCAATGAGA GGCCTGAGGA AAATACCAAT GCTATTGATG GGGCAGGGGA GGAAGCTGAC 3661 TTTCACCAAG CCAGTGCAGA TTTCCGACGG GAGAACATCC TGCCTCACTC TACCTCCAGA 3721 TCCAGGATCA CGTTGCTGTT ACAGTCTCCC CCCGTGAAGT TCCTCATCAG CCCAGAGTTC 3781 TTCACCGTGC TGCATTCTAA CCCTAGTGCC TACCGCATGT TTACAAACAA CACGTGTTTG 3841 AAGCACATGA TCACCAAAGT CCGGAGGGAC ACCCACCACT TTGAACGCTA CCAGCATAAC 3901 CGCGACCTTG TGGGATTCCT CAACATGTTC GCGAACAAAC AGCTAGAGCT GCCGCGGGGA 3961 TGGGAAATGA AACATGATCA CCAGGGCAAG GCATTCTTTG TGGACCACAA CTCCCGCACC 4021 ACCACTTTCA TTGATCCCCG GCTCCCACTT CAGAGCAGTA GACCCACAAG TGCGCTGGTT 4081 CATCGGCAAC ACCTGACAAG GCAACGCAGC CACAGTGCGG GTGAGGTAGG AGAAGATTCT 4141 CGACATGCAG GACCACCAGT TCTTCCCAGG CCATCCAGTA CATTCAATAC AGTCAGTAGG 4201 CCACAGTACC AGGACATGGT TCCAGTGGCT TACAATGACA AGATTGTTGC ATTTCTACGT 4261 CAACCCAATA TCTTTGAAAT TCTACAGGAG CGTCAACCTG ATCTTACCAG GAATCACTCA 4321 CTCAGGGAGA AGATCCAATT TATCCGAACT GAAGGGACTC CAGGATTGGT GCGCCTTTCA 4381 AGCGATGCAG ACCTTGTTAT GTTGCTGAGC TTATTTGAAG AAGAGATAAT GTCGTATGTG 4441 CCTCCACATG CCTTACTCCA CCCCAGCTAC TGTCAGTCCC CACGTGGCTC TCCCGTGTCA 4501 TCTCCTCAGA ACTCGCCAGG TACCCAGCGT GCCAATGCCC GGGCTCCAGC CCCTTACAAG 4561 CGGGATTTCG AAGCCAAACT GAGGAACTTT TACAGGAAGT TAGAGACTAA AGGATATGGA 4621 CAAGGCCCAG GGAAGTTAAA GTTAATTATC CGAAGAGATC ACTTACTAGA AGATGCTTTT 4681 AATCAGATTA TGGGCTACTC CAGAAAAGAC CTGCAGAGAA ATAAGCTATA TGTCACCTTC 4741 GTTGGGGAGG AAGGGCTGGA TTACAGTGGG CCTTCTAGAG AGTTTTTCTT CCTGGTATCC 4801 AGAGAACTCT TTAACCCATA TTATGGCTTA TTTGAATATT CAGCCAATGA CACATACACA 4861 GTACAAATAA GTCCTATGTC TGCTTTTGTA GACAATCACC ATGAATGGTT CCGATTCAGT 4921 GGTAGGATCC TTGGTCTTGC ACTAATACAC CAGTATTTGT TGGATGCCTT CTTCACACGG 4981 CCCTTTTATA AGGCTCTTCT CAGAATTCTA TGTGACCTGA GTGACCTAGA ATACCTTGAT 5041 GAAGAGTTCC ATCAGAGCCT GCAGTGGATG AAAGACAATG ATATCCATGA CATCCTAGAC 5101 CTCACGTTCA CTGTGAACGA AGAAGTATTT GGGCAGATAA CTGAACGAGA ATTAAAGCCA 5161 GGGGGTGCCA ATATCCCAGT TACAGAGAAG AACAAGAAGG AGTACATCGA GAGGATGGTG 5221 AAGTGGAGGA TTGAGAGGGG TGTTGTACAG CAAACAGAGA GCTTAGTGCG TGGCTTCTAT 5281 GAGGTGGTGG ATGCCAGGCT GGTATCTGTT TTTGATGCAA GAGAACTGGA ATTGGTCATC 5341 GCAGGCACAG CTGAAATAGA CCTAAGTGAT TGGAGAAACA ACACAGAATA TAGAGGAGGA 5401 TACCATGACA ATCATATTGT AATTCGGTGG TTCTGGGCTG CAGTGGAAAG ATTCAACAAT 5461 GAACAACGAC TAAGGTTGTT ACAGTTTGTT ACAGGCACAT CCAGCATTCC CTATGAAGGA 5521 TTTGCTTCAC TCCGAGGGAG TAACGGCCCA AGAAGATTCT GTGTGGAGAA ATGGGGGAAA 5581 ATCACTGCTC TTCCCAGAGC GCATACATGT TTTAACCGTC TGGATCTGCC TCCCTACCCA 5641 TCCTTTTCCA TGCTTTATGA AAAACTGTTG ACAGCAGTTG AAGAAACCAG TACTTTTGGA 5701 CTTGAGTGAG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC 5761 GTTTAAACCC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC 5821 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA 5881 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG 5941 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG 6001 GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG 6061 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 6121 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 6181 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 6241 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 6301 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 6361 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 6421 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 6481 AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG 6541 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG 6601 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC 6661 CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC 6721 ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT 6781 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG 6841 CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG 6901 AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG 6961 ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG 7021 GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG 7081 AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG 7141 TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC 7201 TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC 7261 TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC 7321 GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC 7381 AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG 7441 ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT 7501 TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT 7561 TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC 7621 TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT 7681 TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC 7741 ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG 7801 GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC 7861 CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC 7921 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC 7981 TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT 8041 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT 8101 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC 8161 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 8221 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT 8281 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 8341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 8401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 8461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 8521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 8581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 8641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 8701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 8761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 8821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 8881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 8941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 9001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 9061 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 9121 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 9181 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 9241 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 9301 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 9361 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 9421 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 9481 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 9541 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 9601 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 9661 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 9721 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 9781 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 9841 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 9901 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 9961 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 10021 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 10081 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 10141 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.