pLV3-CMV-Samm50-3×FLAG-Puro pl

pLV3-CMV-Samm50-3×FLAG-Puro plasmid

PVT86171

pLV3-CMV-Samm50-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-Samm50(mouse)-3×FLAG-Puro
Alias: NM_178614.5;1110030L07Rik;
Gene lenght: 1407bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Samm50-3×FLAG-Puro plasmid Description

pLV3-CMV-Samm50-3×FLAG-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the mouse Samm50 gene, promoting the C-terminal fusion expression of Samm50 with a 3×FLAG tag, facilitating the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, aiding in the stable integration of the gene into host cells. The plasmid carries a Puro resistance gene for selecting host cells that express the target gene.

pLV3-CMV-Samm50-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC CATGGGGACA GTGCACGCCC GGAGTCTGGA GCCCCTCCCG 2341 TCGAGTGGAA CTGACTTTGG AGCACTGGGG GAGGAAGCCG AGTTTGTGGA AGTTGAGCCG 2401 GAAGCGAAAC AGGAAATCCT GGAAAACAAA GATGTGGTGG TTCAGCATGT TCATTTTGAT 2461 GGACTTGGGC GGACTAAGGA TGACATCATC ATTTGTGAAA TCGGAGAGGT CTTTAAGGCT 2521 AAAAACCTCA TTGAGGTAAT GCGGCGATCT CATGAAGCCC GGGAAAAACT GCTTCGCCTA 2581 GGAATTTTTA GACAAGTGGA TGTTTTGATC GATACATGTC ATGGTGAAGA TGCCCTGCCC 2641 AATGGGTTAG ATGTCACCTT TGAAGTGACA GAGCTGAGGA GACTGACGGG CAGTTACAAC 2701 ACCATGGTTG GAAACAACGA AGGCAGTATG GTACTCGGCC TCAAACTCCC CAACCTTCTG 2761 GGACGAGCAG AAAAAGTCAC TTTCCAGTTT TCTTATGGAA CCAAAGAAAC TTCCTACGGC 2821 CTGTCCTTCT TCAAGCCACA GCCTGGAAAC TTCGAGAGAA ATTTCTCCGT AAACTTATAT 2881 AAAGTTACTG GGCAGTTCCC GTGGAGCTCA CTTCGGGAGA CAGACAGAGG AGTGTCTGCA 2941 GAGTACAGTT TTCCCCTGTG GAAGACCAGT CACACTGTCA AGTGGGAGGG TGTGTGGCGG 3001 GAGCTGGGCT GCCTCTCGAG GACTGCGTCG TTCGCTGTGC GGAAGGAAAG TGGACACTCA 3061 CTGAAGTCGT CTCTCTCGCA TGCCATGGTC ATCGACTCTC GAAATTCATC TATCTTGCCA 3121 AGAAGAGGGG CCTTGTTCAA AGTCAACCAG GAGCTGGCAG GCTACACTGG AGGAGATGTG 3181 AGCTTCATCA AGGAAGACTT TGAGCTTCAG CTGAATAAGC CGCTCGCCTT GGACTCGGTA 3241 TTCTCCACGT CTCTCTGGGG TGGAATGCTG GTGCCCATCG GTGACAAGCC ATCCAGCATT 3301 GCTGACAGGT TTTACCTGGG AGGCCCCACG AGTGTCCGAG GATTTAGCAT GCACAGCATT 3361 GGACCCCAGA GTGAAGGAGA TTACCTGGGC GGCGAGGCCT ACTGGGCTGG GGGCCTGCAC 3421 CTCTACACCC CACTGCCCTT CCGGCCAGGC CAGGGTGGCT TCGGAGAGCT TTTCAGAACT 3481 CACTTTTTCC TCAATGCGGG CAACCTGTGC AACCTCAACT ATGGTGAGGG CCCCAAAGCC 3541 CATATCCGGA AGCTAGCTGA GTGCATCCGC TGGTCCTATG GAGCAGGCGT CGTCCTCCGA 3601 CTTGGCAACA TCGCTCGGCT GGAGCTGAAC TACTGCATTC CTATGGGCGT GCAGGGGGGC 3661 GACAGGATTT GTGATGGTGT CCAGTTTGGA GCTGGGATTC GGTTCCTGGG ATCCGACTAC 3721 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 3781 TAGCGGCCGC GAAGGATCTG CGATCGCTCC GGTGCCCGTC AGTGGGCAGA GCGCACATCG 3841 CCCACAGTCC CCGAGAAGTT GGGGGGAGGG GTCGGCAATT GAACGGGTGC CTAGAGAAGG 3901 TGGCGCGGGG TAAACTGGGA AAGTGATGTC GTGTACTGGC TCCGCCTTTT TCCCGAGGGT 3961 GGGGGAGAAC CGTATATAAG TGCAGTAGTC GCCGTGAACG TTCTTTTTCG CAACGGGTTT 4021 GCCGCCAGAA CACAGCTGAA GCTTCGAGGG GCTCGCATCT CTCCTTCACG CGCCCGCCGC 4081 CCTACCTGAG GCCGCCATCC ACGCCGGTTG AGTCGCGTTC TGCCGCCTCC CGCCTGTGGT 4141 GCCTCCTGAA CTGCGTCCGC CGTCTAGGTA AGTTTAAAGC TCAGGTCGAG ACCGGGCCTT 4201 TGTCCGGCGC TCCCTTGGAG CCTACCTAGA CTCAGCCGGC TCTCCACGCT TTGCCTGACC 4261 CTGCTTGCTC AACTCTACGT CTTTGTTTCG TTTTCTGTTC TGCGCCGTTA CAGATCCAAG 4321 CTGTGACCGG CGCCTACGCT AGATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG 4381 CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC 4441 GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT 4501 CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT 4561 GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC 4621 GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT 4681 GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA 4741 CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG 4801 CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG 4861 GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA GGACCGCGCA CCTGGTGCAT 4921 GACCCGCAAG CCCGGTGCCT GAGTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 4981 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 5041 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 5101 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 5161 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 5221 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 5281 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 5341 GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 5401 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 5461 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT 5521 TTGGGCCGCC TCCCCGCCTG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC 5581 TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAAAT 5641 AAGATCTGCT TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC 5701 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC 5761 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT 5821 AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT 5881 AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGAACTT GTTTATTGCA GCTTATAATG 5941 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 6001 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGCTC TAGCTATCCC 6061 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 6121 TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 6181 TTTTGGAGGC CTAGACTTTT GCAGAGACGG CCCAAATTCG TAATCATGGT CATAGCTGTT 6241 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 6301 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 6361 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC 6421 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 6481 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 6541 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 6601 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 6661 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 6721 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 6781 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 6841 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 6901 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 6961 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 7021 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 7081 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 7141 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 7201 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 7261 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 7321 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 7381 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 7441 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 7501 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 7561 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 7621 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 7681 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 7741 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 7801 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 7861 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 7921 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 7981 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 8041 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 8101 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 8161 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 8221 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 8281 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 8341 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT 8401 TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TCGCGCGTTT 8461 CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT 8521 GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG 8581 TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG 8641 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT 8701 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT 8761 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC 8821 ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.