pCMV-Ncoa1-EGFP-Neo plasmid

pCMV-Ncoa1-EGFP-Neo plasmid

PVT86132

pCMV-Ncoa1-EGFP-Neo plasmid Information

Plasmid name: pCMV-Ncoa1(mouse)-EGFP-Neo
Alias: NM_001408597.1;bHLHe74; KAT13A; mNRC-1; NCoA-1; NRC-1;SRC-1; SRC-a; SRC1;
Gene lenght: 4215bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone: pCMV-MCS-EGFP-Neo
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ncoa1-EGFP-Neo plasmid Description

pCMV-Ncoa1-EGFP-Neo This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a CMV enhancer and a CMV promoter that can jointly initiate the efficient expression of the downstream mouse-derived Ncoa1 gene. The plasmid carries EGFP green fluorescent protein at the C-terminus of the Ncoa1 gene, facilitating the visual observation of the location and level of the target gene expression. The plasmid also carries a Neo/G418 resistance gene, which can be used to select host cells expressing the target gene.

pCMV-Ncoa1-EGFP-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAAGCC ACTGGGATCC GCCACCATGA GTGGCCTTGG GGACAGTTCA TCAGACCCTG 961 CAAACCCAGA CTCTCATAAG AGAAAAGGAT CGCCATGTGA CACGCTGGCA TCAAGTACTG 1021 AGAAGAGGCG CAGGGAGCAA GAAAATAAGT ATTTAGAAGA ACTAGCTGAA CTACTGTCTG 1081 CCAACATCAG TGACATTGAC AGCTTGAGTG TAAAACCAGA TAAATGCAAG ATTTTGAAGA 1141 AGACAGTAGA TCAGATACAG CTAATGAAGA GAATGGAGCA AGAGAAATCC ACGACTGATG 1201 ATGACGTGCA GAAGTCCGAC ATCTCCTCGA GCAGCCAAGG AGTGATAGAG AAGGAGTCGC 1261 TGGGCCCTCT TCTTCTGGAG GCTTTGGATG GATTTTTCTT TGTTGTGAAC TGTGAAGGAA 1321 GAATTGTATT TGTATCAGAA AATGTGACCA GCTACTTGGG TTACAATCAG GAAGAATTAA 1381 TGAATACCAG CGTCTACAGC ATACTGCATG TGGGAGATCA TGCAGAATTT GTCAAGAATC 1441 TGCTACCAAA ATCACTAGTA AATGGAGTTC CTTGGCCTCA AGAGGCAACA CGCCGAAATA 1501 GCCATACATT TAACTGCAGG ATGCTAATTC ACCCTCCAGA GGACCCAGGT ACTGAGAACC 1561 AAGAAGCTTG CCAGCGCTAT GAAGTAATGC AGTGTTTCAC AGTGTCACAG CCAAAATCCA 1621 TCCAAGAAGA TGGTGAAGAT TTCCAGTCAT GTCTGATTTG TATTGCTCGA CGATTACCTC 1681 GGCCTCCAGC CATTACAGGT GTAGAATCCT TTATGACCAA GCAAGATACT ACAGGCAAAA 1741 TCATCTCTAT TGACACTAGC TCCCTGAGGG CTGCTGGCAG GACTGGCTGG GAAGACCTAG 1801 TAAGGAAGTG CATCTATGCT TTCTTCCAAC CTCAGGGCAG AGAGCCATCT TACGCCCGGC 1861 AGCTGTTTCA AGAAGTGATG ACTCGTGGCA CTGCCTCCAG CCCATCCTAT AGATTCATAT 1921 TGAATGATGG GACAATGCTT AGCGCCCACA CCAAGTGTAA ACTTTGCTAC CCTCAAAGTC 1981 CTGACATGCA GCCTTTCATC ATGGGAATTC ATATCATCGA CAGGGAGCAC AGTGGGCTTT 2041 CTCCTCAAGA TGACAGTAAT TCTGGAATGT CAATTCCCCG AATAAATCCC TCAGTCAATC 2101 CTGGTATCTC TCCAGCCCAT GGTGTGACCC GTTCATCCAC GTTGCCACCA TCCAACAACA 2161 ACATGGTCTC TGCCAGAGTA AACCGCCAAC AGAGCTCAGA CCTCAACAGC AGCAGCAGTC 2221 ATACTAACTC TAGCAACAAC CAAGGGAACT TTGGATGTTC ACCTGGAAAT CAGATTGTAG 2281 CCAATGTTGC CTTAAACCAG GGACAGGCAG GTTCCCAGAG CAGCAATCCC TCTTTAAACC 2341 TCAATAATTC TCCTATGGAA GGTACAGGAA TTGCCCTCTC ACAGTTCATG TCTCCGAGGA 2401 GACAAGCTAA TTCTGGCTTG GCAACAAGGG CCAGGATGTC AAACAATTCA TTTCCTCCAA 2461 ATATTCCAAC ATTAAGCTCC CCAGTTGGCA TTACTAGTGG TGCCTGTAAT AATAATAATC 2521 GATCCTATTC AAATATCCCA GTAACATCTT TACAAGGTAT GAATGAAGGA CCCAATAACT 2581 CTGTTGGCTT CTCTGCTGGG TCTCCAGTCC TTCGGCAGAT GAGCTCACAG AATTCACCTA 2641 GCAGATTAAG TATGCAACCA GCAAAGGCTG AGTCCAAAGA CAGCAAAGAG ATTGCATCCA 2701 TTTTAAATGA AATGATTCAG TCCGACAACA GCGACAACAG TGCTAACGAA GGCAAGCCTC 2761 TGGACTCAGG ACTTCTGCAT AACAATGACA GACTCTCAGA AGGAGACAGT AAATACTCTC 2821 AAACTAGTCA CAAGCTAGTA CAGCTATTGA CTACGACCGC AGAGCAGCAG TTACGGCATG 2881 CCGACATAGA CACAAGCTGC AAAGATGTAC TGTCTTGCAC TGGTACTTCC AGCTCTGCCT 2941 CCTCTAACCC CTCAGGAGGT ACTTGTCCCT CTTCTCACAG CTCTCTGACT GAGCGGCATA 3001 AAATTCTGCA CCGGCTTTTA CAGGAGGGCA GCCCCTCAGA CATCACCACT TTGTCTGTGG 3061 AACCCGAGAA GAAGGACAGT GTGCCAGCCT CTACTGCTGT GTCAGTGTCT GGACAATCCC 3121 AGGGGAGTGC CAGCATAAAA CTGGAACTGG ATGCTGCAAA GAAAAAAGAG TCAAAAGACC 3181 ATCAGCTCCT ACGCTACCTT TTAGACAAAG ATGAGAAAGA TTTAAGGTCC ACTCCAAACC 3241 TGTGCCTGGA TGACGTAAAG GTCAAAGTGG AGAAGAAAGA ACAGATGGAT CCTTGTAACA 3301 CAAACCCAAC CCCAATGACC AAACCTGCTC CTGAGGAAGT TAAACTGGAG TCCCAGAGCC 3361 AGTTTACAGC TGACCTTGAC CAGTTTGATC AGTTATTGCC CACCCTGGAG AAGGCAGCAC 3421 AGTTGCCGAG CTTGTGCGAG ACAGACAGGA TGGATGGTGC GGTCACCGGT GTCAGCATCA 3481 AGGCGGAGGT CCTGCCAGCG TCACTCCAGC CCACCACTGC CAGAGCTGCC CCCAGGCTGA 3541 GCAGATTGCC TGAACTAGAA TTAGAAGCGA TCGATAACCA GTTTGGACAA CCAGGAGCGG 3601 GGGATCAGAT TCCATGGGCA AATAATACTT TGACAACAAT AAATCAGAAT AAACCAGAAG 3661 ACCAATGTAT TAGCTCACAG CTGGATGAGC TCCTCTGTCC ACCAACAACA GTAGAAGGAA 3721 GAAACGATGA GAAGGCCCTT CTGGAACAAC TGGTGTCTTT CCTCAGTGGC AAAGATGAAA 3781 CGGAGCTGGC TGAGCTAGAC CGGGCACTGG GGATCGACAA GCTCGTCCAG GGAGGCGGAT 3841 TAGATGTATT ATCTGAGCGA TTTCCACCAC AGCAAGCAAC ACCACCTTTG ATGATGGAAG 3901 ACAGACCCAC CCTCTATTCC CAGCCATACT CATCTCCTTC TCCCACCGCT GGTCTCTCTG 3961 GCCCTTTCCA AGGCATGGTC CGGCAGAAGC CTTCACTGGG GGCTATGCCG GTTCAAGTAA 4021 CACCTCCTCG AGGAACCTTT TCACCCAACA TGGGCATGCA GCCCCGGCAG ACTCTCAATA 4081 GACCTCCAGC TGCACCCAAC CAGCTTCGGC TGCAGCTGCA GCAGCGGCTA CAGGGGCAGC 4141 AGCAGTTGAT GCACCAAAAC CGGCAAGCCA TCTTGAACCA GTTTGCAGCA AATGCTCCTG 4201 TTGGCATGAA CATGAGGTCA GGCATGCAGC AGCAGATCAC ACCTCAGCCA CCCTTGAATG 4261 CTCAGATGTT GGCCCAGCGC CAGCGGGAGT TGTACAGTCA GCAGCATCGA CAGAGGCAAA 4321 TTATACAGCA GCAGAGAGCC ATGCTCATGA GGCACCAAAG TTTTGGGAAC AACATCCCTC 4381 CTTCATCTGG ACTCCCAGTT CAAATGGGGA CTCCCCGTCT TCCTCAGGGT GCTCCCCAGC 4441 AGTTCCCCTA CCCACCAAAC TATGGTACAA ACCCAGGAAC CCCACCTGCC TCCACCAGCC 4501 CTTTCTCTCA ACTGGCAGCA AACCCTGAGG CCTCCTTGGC CACCCGCAGC AGCATGGTGA 4561 ACAGAGGCAT GGCAGGGAAC ATGGGAGGAC AGTTTGGCGC TGGCATCAGT CCTCAGATGC 4621 AGCAGAATGT CTTCCAATAT CCAGGACCAG GACTAGTTCC CCAAGGTGAG GCCACCTTTG 4681 CCCCATCTCT AAGCCCTGGG AGCTCCATGG TGCCGATGCC AGTCCCTCCT CCTCAAAGCT 4741 CTCTGCTCCA GCAAACTCCA CCTACTTCTG GGTACCAGTC ACCAGACATG AAGGCCTGGC 4801 AGCAAGGAAC AATGGGAAAC AACAATGTGT TCAGTCAAGC TGTCCAGAGC CAGCCTGCAC 4861 CTGCACAGCC AGGAGTGTAC AACAACATGA GCATCACCGT GTCCATGGCA GGTGGAAACG 4921 CAAACATTCA GAACATGAAT CCAATGATGG GCCAGATGCA AATGAGCTCT CTGCAGATGC 4981 CAGGGATGAA TACTGTGTGC TCTGAGCAGA TGAATGATCC AGCACTGAGA CACACAGGCC 5041 TCTACTGCAA CCAGCTCTCG TCCACTGACC TTCTCAAAAC AGACGCAGAT GGAAACCAGG 5101 ACAAGAAGAC AGAAGAGTTC TTCTCTGTGG TGACTACAGA CGGCTCGAGT GGCGGTGGAG 5161 GAAGCGGCGG AGGTGGATCT ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC 5221 CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG 5281 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC 5341 TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC 5401 GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG 5461 TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA 5521 AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG 5581 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA 5641 TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG 5701 ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG 5761 TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG 5821 AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA 5881 TGGACGAGCT GTACAAGTAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG 5941 TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG 6001 AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA 6061 GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG 6121 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA 6181 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 6241 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 6301 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 6361 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 6421 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 6481 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 6541 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 6601 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT 6661 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 6721 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 6781 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT 6841 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC 6901 AGAGGCCGAG GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG 6961 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA 7021 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC 7081 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC 7141 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA 7201 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC 7261 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT 7321 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA 7381 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC 7441 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT 7501 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC 7561 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG 7621 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT 7681 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT 7741 TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA 7801 GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA 7861 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC 7921 TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC 7981 GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT 8041 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT 8101 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT 8161 AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 8221 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 8281 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 8341 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 8401 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 8461 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 8521 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 8581 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 8641 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 8701 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 8761 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 8821 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 8881 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 8941 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 9001 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 9061 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 9121 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT 9181 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 9241 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 9301 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 9361 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 9421 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 9481 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 9541 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 9601 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 9661 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 9721 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 9781 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 9841 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 9901 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 9961 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 10021 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 10081 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 10141 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 10201 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 10261 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 10321 AAAGTGCCAC CTGACGTC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.