pLV3-CMV-Srp68-3×FLAG-CopGFP-P

pLV3-CMV-Srp68-3×FLAG-CopGFP-Puro plasmid

PVT86097

pLV3-CMV-Srp68-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Srp68(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_146032.3;2610024I03Rik
Gene lenght: 1875bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone: pLV3-CMV-MCS-3×FLAG-CopGFP-Puro
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Srp68-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Srp68-3×FLAG-CopGFP-Puro This plasmid is suitable for overexpression in mammalian cells. The plasmid contains a strong CMV promoter that can initiate the transcription of the mouse Srp68 gene, promoting the C-terminal fusion expression of the Srp68 gene with a 3×FLAG tag, facilitating the detection, localization, and purification of the expressed fusion protein using FLAG antibodies. Additionally, the plasmid carries HIV-1 Ψ and 3'/5' LTR elements for lentivirus packaging, facilitating the stable integration of the gene into host cells. The CopGFP green fluorescent protein carried by the plasmid is used to observe the expression and localization of the target gene. The plasmid carries a Puro resistance gene for selecting host cells that express the target gene.

pLV3-CMV-Srp68-3×FLAG-CopGFP-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT 1921 TATCGATACT AGTATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC 1981 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG 2041 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG 2101 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA 2161 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTTT ATATAAGCAG AGCTCGTTTA 2221 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGATTC 2281 TAGAGCTAGC CTGATACGAA CTCGGAATTC GCCACCATGG CTGCTGAGAA GCAGATCCCG 2341 GGTGGTGGTA GTGGTGGTGG TGGAAGCGGC AGCGGCGGCG GCGGCGGAGG GAGCGGTGGC 2401 GGACGCAGTG CCGGAGGAGA CGAGAATAAA GAAAACGAAC GCCCTTCAGC CGGATCGAAG 2461 GCAAACAAAG AGTTCGGGGA CAGCCTGAGT TTGGAGATTC TGCAGATCAT TAAGGAGTCG 2521 CAGCAGCAGC ATGGCTTACG GCACGGAGAC TTCCAGCGGT ACAGGGGCTA CTGTTCTCGA 2581 AGACAAAGAC GCCTCCGGAA AACACTCAAC TTTAAGATGG GGAACAGACA CAAATTCACG 2641 GGGAAGAAAG TAACGGAGGA GCTCCTGACT GATAACAGAT ACTTGCTGCT GGTGCTGATG 2701 GATGCAGAAA GAGCCTGGAG CTACGCCATG CAGCTCAAAC AGGAAGCCAA CACCGAGCCG 2761 CGGAAACGGT TTCACTTGCT GTCTCGCCTT CGAAAAGCCG TGAAGCATGC AGAGGAACTG 2821 GAGCGCTTGT GTGAGAGCAA CCGGGTGGAT GCCAAGACCA AGCTAGAGGC TCAGGCCTAC 2881 ACGGCTTACC TCTCAGGAAT GTTGCGCTTT GAACATCAAG AGTGGAAATC TGCCATTGAG 2941 GCTTTCAACA AATGCAAAAC TATCTATGAG AAGCTAGCCA GTGCATTCAC AGAGGAGCAG 3001 GCTGTGTTGT ACAACCAGCG TGTAGAAGAG ATCTCGCCCA ACATCCGCTA CTGTGCATAC 3061 AACATTGGGG ACCAGTCAGC TATCAATGAG CTCATGCAGA TGAGACTAAG GTCTGGGGGC 3121 ACAGAGGGGC TCCTTGCTGA AAAACTTGAG GCCTTGATCA CTCAGACTCG AGCCAAGCAG 3181 GCGGCGACCA TGAGTGAGGT GGAGTGGCGA GGCCGGACGG TCCCCGTGAA GATCGACAAA 3241 GTGAGGATCT TCCTCCTGGG CCTGGCCGAC AACGAGGCCG CCATCGTCCA GGCTGAAAGT 3301 GAAGAAACGA AGGAGCGTCT GTTCGAGTCC ATGCTGAGTG AGTGCCGAGA CGCCCTCCAA 3361 GCCGTGAGGG AAGAGCTCAA GCCCGATCAG AAGCAGAGAG ACTATGCCCT TGATGGAGAG 3421 TCTGGGAAGG TGTCAAATCT CCAGTACCTG CACAGCTACC TGACCTACAT CAAGCTGTCA 3481 ACAGCAATCA GGCGAAACGA GAACATGGCT AAAGGCTTGC ACAGGGCTCT GCTGCAGCAG 3541 CAGCCGGAAG ATGACAGCAA GCGCTCTCCC AGGCCTCAGG ACCTCATCCG GCTCTATGAC 3601 ATCATTCTGC AGAATCTGGT GGAATTGCTG CAGCTTCCTG GCTTAGAAGA GGACAGAACC 3661 TTCCAGAAAG AGATAAGCCT CAAGACCCTG GTGTTCAAAG CTTACAGGTG TTTCTTCATT 3721 GCTCAGTCTT ACGTGCTGGT GAAGAAGTGG AGCGAAGCCC TCGTCCTGTA CGACAGAGTT 3781 CTCAAATATG CAAATGAAGT GAGTTCTCAC GGAGGAGCCT CCAAGAACAG CCTGAAGGAC 3841 CTGCCCGACG TGCAAGAACT CATCACTCAA GTGCGGTCAG AGAAGTGCTC GCTGCAGGCC 3901 GCAGCCATCC TGGATGCAAA CGACTCTCAT CAAACAGACA CCTCATCCCA AGTCAAAGAC 3961 AATACGCCTC TGGTTGAACG GTTTGAGTCC TTTTGCCTGG ACCCGTCCCT GGTCACCAAA 4021 CAAGCCAACC TCGTGCACTT CCCACCAGGG TTCCAGCCCA TCCCTTGCAA GCCTTTGTTC 4081 TTTGACCTGG CCCTCAACCA CGTGGCCTTC CCACCCCTTG AGGACAAATT GGAGCAGAAG 4141 ACTAAGAGTG GCCTCACAGG CTACATCAAG GGCATCTTTG GTTTCAGGAG CGGATCCGAC 4201 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 4261 AAGTAGCGGC CGCGAAGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC AGAGCGCACA 4321 TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG TGCCTAGAGA 4381 AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT TTTTCCCGAG 4441 GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT TCGCAACGGG 4501 TTTGCCGCCA GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC ACGCGCCCGC 4561 CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC TCCCGCCTGT 4621 GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC GAGACCGGGC 4681 CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC GCTTTGCCTG 4741 ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG TTACAGATCC 4801 AAGCTGTGAC CGGCGCCTAC GCTAGACGCC ACCATGGAGA GCGACGAGAG CGGCCTGCCC 4861 GCCATGGAGA TCGAGTGCCG CATCACCGGC ACCCTGAACG GCGTGGAGTT CGAGCTGGTG 4921 GGCGGCGGAG AGGGCACCCC CAAGCAGGGC CGCATGACCA ACAAGATGAA GAGCACCAAA 4981 GGCGCCCTGA CCTTCAGCCC CTACCTGCTG AGCCACGTGA TGGGCTACGG CTTCTACCAC 5041 TTCGGCACCT ACCCCAGCGG CTACGAGAAC CCCTTCCTGC ACGCCATCAA CAACGGCGGC 5101 TACACCAACA CCCGCATCGA GAAGTACGAG GACGGCGGCG TGCTGCACGT GAGCTTCAGC 5161 TACCGCTACG AGGCCGGCCG CGTGATCGGC GACTTCAAGG TGGTGGGCAC CGGCTTCCCC 5221 GAGGACAGCG TGATCTTCAC CGACAAGATC ATCCGCAGCA ACGCCACCGT GGAGCACCTG 5281 CACCCCATGG GCGATAACGT GCTGGTGGGC AGCTTCGCCC GCACCTTCAG CCTGCGCGAC 5341 GGCGGCTACT ACAGCTTCGT GGTGGACAGC CACATGCACT TCAAGAGCGC CATCCACCCC 5401 AGCATCCTGC AGAACGGGGG CCCCATGTTC GCCTTCCGCC GCGTGGAGGA GCTGCACAGC 5461 AACACCGAGC TGGGCATCGT GGAGTACCAG CACGCCTTCA AGACCCCCAT CGCCTTCGCC 5521 AGATCCCGCG CTCAGTCGTC CAATTCTGCC GTGGACGGCA CCGCCGGACC CGGCTCCACC 5581 GGATCTCGCG AGGGCAGAGG AAGTCTTCTA ACATGCGGTG ACGTGGAGGA GAATCCCGGC 5641 CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC 5701 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG 5761 GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC 5821 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG 5881 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC 5941 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG 6001 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG 6061 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC 6121 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC 6181 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC 6241 TGAAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 6301 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 6361 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 6421 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 6481 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 6541 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 6601 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 6661 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 6721 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 6781 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGTACC 6841 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 6901 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGATAAGAT CTGCTTTTTG CTTGTACTGG 6961 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 7021 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 7081 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 7141 TAGTAGTTCA TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA 7201 GAGTGAGAGG AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 7261 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 7321 CAATGTATCT TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CATCCCGCCC 7381 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 7441 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 7501 GGAGGCCTAG ACTTTTGCAG AGACCAAATT CGTAATCATG TCATAGCTGT TTCCTGTGTG 7561 AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC 7621 CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT 7681 CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 7741 CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT 7801 TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 7861 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 7921 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 7981 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 8041 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 8101 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 8161 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 8221 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 8281 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 8341 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG 8401 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 8461 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 8521 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 8581 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 8641 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 8701 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 8761 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 8821 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 8881 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 8941 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 9001 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 9061 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 9121 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 9181 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 9241 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 9301 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 9361 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 9421 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 9481 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 9541 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 9601 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 9661 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC 9721 ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA 9781 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA 9841 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG 9901 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT 9961 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG 10021 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 10081 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 10141 TAAAACGACG GCCAGTGCCA AGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.