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上海玉博生物科技有限公司
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文献和实验, to be used for RNA or DNA FISH, are labeled by nick translation using 1 to 2µg of DNA per 50µl of reaction and following manufacturer's instructions (see note 4); Approximately 0.1µg of probe (usually 5µl of a standard nick translation reaction of 50µ
-hybridization wash (50% formamide/2X SSC): To make 500 ml, take 250 ml formamide, add 50 ml 20X SSC stock and complete to 500 ml with DDW. 3. Probe competitor solution (ssDNA/tRNA): To make 100 µl of 10 mg/ml total concentration competitor, take 50 µl of 10
-hybridization wash (50% formamide/2X SSC): To make 500 ml, take 250 ml formamide, add 50 ml 20X SSC stock and complete to 500 ml with DDW. 3. Probe competitor solution (ssDNA/tRNA): To make 100 µl of 10 mg/ml total concentration competitor, take 50
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