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文献和实验Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
and stability. Here we elaborate detailed protocols using maleimide-based chemistry for the conjugation to reduce hinge disulfides in antibodies by several cytotoxic drugs. We present a method for the initial characterization of the reduction/alkylation reaction
while vortexing. If Biotinylating, add 150uL of Biotin/Water solution to Antibody while vortexing. 3. Incubate at room temperature for 45minutes. 4. The next step is to separate the coupled from uncoupled FITC or Biotin. This can be done in 2 ways
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