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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Stable for 1 year at 4°C from date of receipt
- 英文名:
HA Tag CUTANA™ CUT&RUN Antibody
- 库存:
99
- 供应商:
欣博盛生物
- 规格:
100 µg
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文献和实验Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Protocol for anti-HA antibody Western Blotting
your antibody) 5) Incubate in primary antibody solution: I use PBS with 0.1% TWEEN-20 plus 5% BSA as the basic solution, but you can use TBS too. I dilute the HA antibody 1:5000, just to be sure you will see a signal. I haven't EVER seen appreciable
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
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