- ￥1274
- Cayman
- 36976-25 µg
- 2026年03月09日
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文献和实验E.Z.N.Z.TM MicroElute RNA Clean-up Protocol
实验步骤 1. Measure the volume of sample and adjust the sample volume to 100ul with DEPC-Water and proceed to step 2. 2. Add 350ul QVL Lysis buffer and mix by vortexing at maximum speed for 15 seconds. When process small
E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol
(supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection tube. 5. Place column in the same 2 ml collection tube from step 4 and add 500 ul RWB Wash Buffer diluted with absolute
Lac-Z Detection in Tail Biopsies
2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X PBS solution over biopsy with 2.5% Glutaraldehyde diluted in SpM buffer
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