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- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
ORC-2
- 库存:
999
- 供应商:
博奥派克
- 规格:
Supernatant 1ml
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文献和实验Replication Initiation Point Mapping: Approach and Implications
is a sensitive technique and has been successfully applied to single copy loci in both budding and fission yeast, archaebacteria, and human cells. Studies in yeast have shown that the binding site for the initiator, the origin recognition complex (ORC), lies
ChIP-CpG Island Microarray Binding Analysis
machinery, such as ORC and MCMs.In certain cases, the investigators have monitored all intergenic regions and/or known promoters in the yeast genome. In other cases, the authors have taken advantage of the relatively small size of the yeast genome to spot
弄明白的领域。在报告里面,他特别提到了表达纯化CDC6蛋白的经历,我觉得十分有意思。这个蛋白, 用他的话说,是\"a pain in the ass\"。 CDC6是干什么的呢?这要从ORC说起。真核生物的DNA有多个复制起始位点 (replication origin),ORC(Origin Replication Complex)是一个很大的蛋白复合体,可以识别这些复制起始位点。ORC在整个细胞周期里面都是与DNA结合的。在G1期里面,CDC6这个蛋白会与ORC结合,同时让MCM
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