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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
WB,IHC-P,ICC/IF
- 适应物种:
Human,Mouse,Rat
- 标记物:
Unconjugated
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 形态:
liquid
- 规格:
50μl/100μl
| 规格: | 50μl | 产品价格: | ¥1024.0 |
|---|---|---|---|
| 规格: | 100μl | 产品价格: | ¥1824.0 |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
,EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体,而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体:hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本:蜡包埋人类肺部肿瘤抗原修复缓冲液:柠檬酸盐缓冲液(左)或者 EDTA 缓冲液(右)煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后,才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然,一些研究人员也使用水浴锅,但是,因为该步骤中所选择的装置对染色存在积极或消极的影响
) is accompanied with or inactivates the GSK activity. However, it is still not conclusive what the role of Tyr phosphorylation (Y216 fro beta, Y279 for alpha) on GSK activity is. One reference is a review paper published in Cell in 2001, you may take a quick
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