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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
WB,IHC-F,ICC/IF,FC,Functional Assay
- 适应物种:
Mouse
- 标记物:
Unconjugated
- 保存条件:
SStore at 4°C.Avoid freeze/thaw cycles.
- 形态:
Liquid
- 规格:
1mg
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文献和实验saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Fusion of Mouse, Rat, or Hamster Cell
the pellet in HAT medium to 5 x 105 cells/ml. Plate 200 ml/well to 96 well plates. Incubate at 37o C, 8-10 CO2 for 5-7 days.
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