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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海西格
- 库存:
36
- 靶点:
详见说明书
- 级别:
详见说明书
- 目录编号:
详见说明书
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 抗体英文名:
Anti-nitrated Alpha-synuclein (nitro-Tyr39)
- 抗体名:
硝基化α-突触核蛋白抗体
- 标记物:
详见说明书
- 宿主:
详见说明书
- 适应物种:
详见说明书
- 免疫原:
KLH conjugated Synthesised nitrylpeptide derived from human Alpha-synuclein around the nitrosation site of Tyr39
- 亚型:
IgG
- 形态:
详见说明书
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 浓度:
1mg/1ml
- 保存条件:
详见说明书
(1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
(2)活补体:IgM、IgG1、IgG2和IgG3可通过经典途径激活补体,凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
(3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
(4)可通过胎盘及粘膜:免疫球蛋白G(IgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白A(IgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
(5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
(6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,60~70℃即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。
| 产品名称 | 硝基化α-突触核蛋白抗体 |
| 英文名称 | Anti-nitrated Alpha-synuclein (nitro-Tyr39)抗体 |
| 货号 | XGK0225 |
中文名称 硝基化α-突触核蛋白抗体
别 名 SYUA_HUMAN; nitrated Alpha-synuclein (nitrated-Tyr39); nitrated Alpha-synuclein (nitro-Tyr39); nitrated Alpha synuclein; nitrated Alpha-Synuclein; Alpha synuclein; Alpha-synuclein, isoform NACP140; alphaSYN; MGC105443; MGC110988; MGC127560; MGC64356; NACP; Non A beta component of AD amyloid; Non A4 component of amyloid precursor; Non-A-beta component of alzheimers disease amyloid , precursor of; PARK 1; PARK 4; PARK1; PARK4; Parkinson disease familial 1; PD 1; PD1; SNCA; Snca synuclein, alpha (non A4 component of amyloid precursor); Synuclein alpha; Synuclein, alpha (non A4 component of amyloid precursor).
浓 度 1mg/1ml
规 格 0.1ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig
产品类型 一抗
研究领域 细胞生物 神经生物学 细胞凋亡
蛋白分子量 predicted molecular weight: 15kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised nitrylpeptide derived from human Alpha-synuclein around the nitrosation site of Tyr39
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
产品介绍 The synuclein family members, including Alpha-synuclein (also designated NACP for non-Beta-Amyloid component) and Beta-synuclein, are predominantly expressed in the brain and are speculated to be involved in synaptic regulation and neuronal plasticity. Alpha-synuclein is localized to neuronal cell bodies and synapses. Alpha-synuclein was first identified as a component of Alzheimer’s disease amyloid plaques. Abnormal platelet function in Alzheimer’s disease has been demonstrated. During megakaryocytic differentiation Alpha-synuclein has been found to be upregulated, while Beta-synuclein is downregulated, indicating that coordinate expression of synucleins may be important during hematopoetic cell differentiation. A mutant form of Alpha-synuclein has been found in patients with early onset Parkinson’s disease.
Function : May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.Subunit : Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones.Subcellular Location : Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Note=Membrane-bound in dopaminergic neurons.Tissue Specificity : Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.Post-translational modifications : Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.Ubiquitinated. The predominant conjugate is the diubiquitinated form.DISEASE : Note=Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.Defects in SNCA are the cause of Parkinson disease type 1 (PARK1) [MIM:168601]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features are characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. The disease is progressive and usually manifests after the age of 50 years, although early-onset cases (before 50 years) are known. The majority of the cases are sporadic suggesting a multifactorial etiology based on environmental and genetic factors. However, some patients present with a positive family history for the disease. Familial forms of the disease usually begin at earlier ages and are associated with atypical clinical features.Defects in SNCA are the cause of Parkinson disease type 4 (PARK4) [MIM:605543]. A complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.Defects in SNCA are the cause of dementia Lewy body (DLB) [MIM:127750]. A neurodegenerative disorder clinically characterized by mental impairment leading to dementia, parkinsonism, often with fluctuating cognitive function, visual hallucinations, falls, syncopal episodes, and sensitivity to neuroleptic medication. Brainstem or cortical intraneuronal accumulations of aggregated proteins (Lewy bodies) are the only essential pathologic features. Patients may also have hippocampal and neocortical senile plaques, sometimes in sufficient number to fulfill the diagnostic criteria for Alzheimer disease.Similarity : Belongs to the synuclein family.
抗体制备过程:
1.材料与试剂
a.提取的动物 Ig
b.弗氏完全佐剂和弗氏不完全佐剂
c.青霉素和链霉素
d.实验动物 兔
e.其它材料及试剂
2、选择实验活体。
3、进行动物免疫实验。
4、试取血样进行测试,查看免疫效果。
5、如果免疫成功,杀死实验活体,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。胎牛血清(无菌采制)
我司提供以下技术外包服务:
1. Anti-nitrated Alpha-synuclein (nitro-Tyr39)抗体分子生物学:质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学:基因合成、引物合成、基因测序、载体构建等
2.蛋白工程:原核、哺乳动物蛋白表达系统等
3.病毒包装:腺病毒、慢病毒等
4.抗体工程:磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等
5.细胞工程:细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。
Anti-OVA/HRP 辣根过氧化物酶标记兔抗鸡卵白蛋白抗体IgGMulti-class antibodies规格: 0.2ml
PACAP-38 腺苷酸环化酶激活肽-38(抗原)Multi-class antibodies规格: 0.5mg
胸腺肽 α-1抗体 Anti-Thymosin α-1 0.1ml
S100 alpha 2 英文名称: S100A2抗体 0.2ml
EAAT3 英文名称: 胶质细胞谷酸运载蛋白3抗体(神经/上皮细胞谷酸运载蛋白) 0.1ml
Rhesus antibody Rh Phospho-KSR1 (Ser392) 0酸化Ras信号转导KSR1蛋白抗体 规格 0.1ml
PACAP-38 腺苷酸环化酶激活肽-38(抗原)Multi-class antibodies规格: 0.5mg
Anti-PRDX3/FITC 荧光素标记过氧化还原酶3抗体IgGMulti-class antibodies规格: 0.2ml
Rabbit Anti-horse IgG Whole serum 兔抗马IgG抗血清Multi-class antibodies规格: 1ml
拟南芥IKI3抗体 Anti-IKI3 family protein 0.2ml
Donkey anti-Gpig IgG whole serum 驴抗豚鼠IgG抗血清 1ml
FAKD3 英文名称: 下颌发育不良综合征相关蛋白FAKD3抗体 0.2ml
Rhesus antibody Rh phospho-PTPN7(Ser93) 0酸化非受体型蛋白酪氨酸0酸酶7抗体 规格 0.1ml
Rabbit Anti-horse IgG Whole serum 兔抗马IgG抗血清Multi-class antibodies规格: 1ml
phospho-NFKB p65 (pSer536) peptide 0酸化细胞核因子/0酸化k基因结合核因子抗原Multi-class antibodies规格: 0.5mg
Anti-APOD 载脂蛋白D抗体Multi-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-ATXN1(Thr236) 0酸化失调症蛋白1抗体 规格 0.1ml
山羊抗兔 IgG(H+L)/Biotin 0.1ml 美国Jackson公司分装
VEGF-A 英文名称: 血管内皮生长因子A 0.1ml
DNA polymerase delta 英文名称: DNA聚合酶γ抗体 0.2ml
Anti-APOD 载脂蛋白D抗体Multi-class antibodies规格: 0.2ml
人促胰液素/分泌素受体(SR)ELISA 试剂盒 96T/48T 试剂盒 组装/原装
小鼠25羟基胆固醇(25-OHC)免疫试剂盒 Mouse 25-hydroxycholesterol,25-OHC ELISA kit
天门冬酰-NA合成酶(DARS)ELISA试剂盒 ,英文名: DARS ELISA Kit
MouseSerumamyloidA,SAAELISA试剂盒小鼠血清淀粉样蛋白A(SAA)ELISA试剂盒规格:96T/48T
ChickenIerleukin9,IL-9ELISA试剂盒鸡白介素9(IL-9)ELISA试剂盒规格:96T/48T
HumanAcrineOrange,AOELISAKit人橙(AO)ELISA试剂盒规格:96T/48T
Anti-nitrated Alpha-synuclein (nitro-Tyr39)抗体人巨噬细胞炎性蛋白5(MIP-5)ELISA 试剂盒 96T/48T 试剂盒 组装/原装
小鼠核因子κB(NF-κB)免疫试剂盒 Mouse Nuclear factor-kappa B,NF-κB ELISA Kit
胰岛素受体底物2(IRS-2)ELISA试剂盒 ,英文名: IRS-2 ELISA Kit
MouseApolipoproteinH,Apo-HELISA试剂盒小鼠载脂蛋白H(Apo-H)ELISA试剂盒规格:96T/48T
ELISAKitCys-C小鼠半胱氨酸蛋白酶抑制剂/胱抑素C规格:48T/96T
GuineaPigcalcitoningenerelatedpeptide,CGRPELISAKit豚鼠降钙素基因相关肽(CGRP)ELISA试剂盒规格:96T/48T
抗体的鉴定:
1)抗体的效价鉴定:鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
3)真核翻译起始因子2C2抗体的亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。
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文献和实验人抗狂犬病毒抗体(anti-RV)酶联免疫分析(ELISA)
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david9931 请问各位大侠,有没有订做过磷酸化的丝氨酸或磷酸化的苏氨酸抗体,哪家公司做的比较好,小弟想订做目的蛋白特异位点的丝氨酸、苏氨酸的磷酸化的抗体,帮忙给指点下,多谢啦 euthor_hsu 这种抗体定做很难。毕竟要的是抗一个特异的氨基酸的磷酸化位点。 就我所知cisbio有anti-phosphothronine 和anti-phosphotyrosine的抗体,PAb和MAb都有,但是没有anti
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