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文献和实验channels (Steps 40�43). Alternatively, it is possible to attach endosomes to coverslips using other approaches such as dilution in low-melt agarose, which is then allowed to harden on the coverslips. For the docking/fusion assay, double-labeled ('yellow
Th17 Polarization of Mouse CD4 Cells
Anti-mouse CD28, clone37.51[Details: LEAF™format, cat.#102112] Anti-mouse IL-4 [Details: LEAF™format, cat#504108] Anti-mouse IFN-γ [Details: LEAF™format, cat.#505812] Recombinant mouse IL-6(carrier-free)[Details: Biolengend, cat
supply companies and is a JT Baker reagent, cat # 4730-02 (50% concentrate). The table below is used by our lab, and is sufficient for a standard 35 x 43 cm x 0.4 mm gel (we use the Owl boxes). The Long Ranger solution comes with a troubleshooting
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