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文献和实验CAT ASSAY[TLC METHOD] 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS
CAT ASSAY[TLC METHOD] 1. Transfer cells to a 15 ml tube. 2. Add 5 ml TBS- to flasks, shake & pour into tubes. 3. Spin down the cells @ 1k rpm for 5'. 4. Resuspend in 1 ml TBS- . 5. Transfer to 1.5 ml tubes. 6. Spin down @ 14K RPM
Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
reporter genes can be used to measure transcription activity, such as chloramphenicol acetyl transferase (CAT), luciferase, (β-galactosidase, and human growth hormone (7 ). To examine the transcription enhancement activity of a putative promoter/enhancer
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