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- 文献和实验
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- 保存条件:
-20℃
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- 英文名:
CNEE (rPeptide, Cat#N-1062-1)
- 供应商:
rPeptide
- 规格:
5mg
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文献和实验Recipes for Tissue Culture Selective Drug Stocks
): Obtained as crystalline powder from Sigma (cat.# A-4882, listed as 2-amino-6-mercaptopurine) and stored @ room temp. in T932. C5H5N5S FW=167.2. The 1000X stock solution is prepared in PBS (alkalinated with NaOH) to give a final stock
In Situ Hybridization Using Digoxigenin Labeled Probes
the chromosomes by incubation in freshly prepared 0.07 N NaOH for 3 minutes 6) Wash the slides in 2X SSC two times for 5 minutes each 7) Ethanol dehydrate two times 5 minutes in 70% ethanol and 5 minutes in 95% ethanol. Air dry. Random Primed DNA Labeling
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Direct labeling of total RNA with Cy3 and Cy5: A. MATERIALS RNeasy® Mini Kit (Qiagen; Cat # 74106) SuperScript II RT (200U/µL) (Life Technologies; Cat # 18064-014) QIAquick PCR Purification Kit (Qiagen; Cat # 28106)
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