- ￥693.50 - 3467.50
- Enzo
- ALX-380-013
- 美国
- 2026年03月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
少量现货
- 英文名：
G418 . sulfate
- CAS号：
108321-42-2
- 保质期：
As indicated on product label or CoA when stored as recommended. Solutions are stable for 3 to 6 months when stored at -20°C.
- 供应商：
欣博盛生物
- 保存条件：
-20°C
- 规格：
1 g/5 g/500 mg
| 规格： | 1 g | 产品价格： | ￥915.42 |
|---|---|---|---|
| 规格： | 5 g | 产品价格： | ￥3467.5 |
| 规格： | 500 mg | 产品价格： | ￥693.5 |
G418 . sulfate产品货号ALX-380-013，由ENZO公司研发生产，欣博盛生物是ENZO公司授权一级代理商，如需购买ENZO公司产品，技术问题，请联系欣博盛生物。热销品常备现货~
Antibiotic
Used to select neoR-positive cell lines and plasmids
Highly cited
G418 is a gentamycin-class aminoglycoside antibiotic with cytotoxic properties in both prokaryotic and eukaryotic cells. In a cell-free assay, G418 inhibits 80S ribosome and protein elongation during translation. In cells, G418 induces apoptosis and is widely employed in the selection of eukaryotic expression vectors containing the neomycin resistance gene neoR, in combination with either APH(2”) or APH(3′).
This product is the disulfide salt form of G418 CAS# 49863-47-0.
Product Details
Activity
Potency: ≥700 µg/mg
Alternative Name
Geneticin
Appearance
White to off-white powder.
CAS
108321-42-2
Formula
C20H40N4O10 . 2H2SO4
Identity
Identity determined by HPLC and IR.
MW
496.6 . 196.1
RTECS
CB9378500
Solubility
Soluble in water.
Source
Isolated from Micromonospora rhodorangea.
Technical Info / Product Notes
The effective killing concentration of this antibiotic will vary as to the cell type, media, growth conditions, and the cells metabolic rate and position in the cell cycle. Effective concentrations have been reported from 100µg/ml up to 5mg/ml or greater. When using G418 in a new cell system, a full dose curve is suggested and with each new lot of G418, several points on that curve should be retested, as the potency determined in a standard B. subtilis assay may not exactly correlate to your system.
Handling & Storage
Use/Stability
As indicated on product label or CoA when stored as recommended. Solutions are stable for 3 to 6 months when stored at -20°C.
Handling
Avoid freeze/thaw cycles.
Long Term Storage
-20°C
Shipping
Ambient Temperature
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文献和实验A GluN2B disease-associated variant promotes degradation of NMDA receptors via autophagy: Benske, T. M., Williams, M. P., et al.; bioRxiv , (2025)
KIF2C-induced nuclear condensation concentrates PLK1 and phosphorylated BRCA2 at the kinetochore microtubules in mitosis: Skobelkina, A., Julien, M., et al.; bioRxiv , (2024)
Comparison of mutations in human parainfluenza viruses during passage in primary human bronchial/tracheal epithelial air-liquid interface cultures and cell lines: Sugimoto, S., Kawase, M., et al.; Microbiol. Spectr. 12, e0116424 (2024), Abstract
Nanoparticles Based on Natural Lipids Reveal Extent of Impacts of Designed Physical Characteristics on Biological Functions: N. Kimura, et al.; ACS Nano 18, 1432 (2024), Abstract
The endoplasmic reticulum membrane complex promotes proteostasis of GABAA receptors: A.L. Whittsette, et al.; iScience 25, 104754 (2022), Abstract — Full Text
Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABAA receptors: M. Wang, et al.; Cell Biosci. 12, 48 (2022), Abstract — Full Text
Preclinical studies of the triazolo[1,5-a]pyrimidine derivative WS-716 as a highly potent, specific and orally active P-glycoprotein (P-gp) inhibitor: S.Q. Wang, et al.; Acta Pharm. Sin. B 12, 3263 (2022), Abstract
Overexpression of ABCC1 Confers Drug Resistance to Betulin: X. Chen, et al.; Front. Oncol. 11, 640656 (2021), Application(s): Cell treatment (carcinoma cell lines: HEK293), Abstract — Full Text
Proteostasis regulators restore function of epilepsy-associated GABAA receptors: X. Di, et al.; Cell Chem. Biol. 28, 46 (2021), Abstract — Full Text
Pharmacokinetic engineering of OX40-blocking anticalin proteins using monomeric plasma half-life extension domains: M. Siegemund, et al.; Front. Pharmacol. 12, 759337 (2021), Abstract — Full Text
Inhibition of gastric H+,K+-ATPase by new dihydropyrazole derivative KYY-008: T. Fujii, et al.; Biochem. Biophys. Res. Commun. 567, 177 (2021), Abstract
Transcriptional suppression of ribosomal DNA with phase separation: S. Ide, et al.; Sci. Adv. 6, 42 (2020), Abstract — Full Text
IP3 receptor isoforms differently regulate ER-mitochondrial contacts and local calcium transfer: A. Bartok, et al.; Nat. Commun. 10, 3726 (2019), Abstract — Full Text
Single nucleosome imaging reveals loose genome chromatin networks via active RNA polymerase II: R. Nagashima, et al.; J. Cell. Biol. 218, 1511 (2019), Abstract — Full Text
Remodeling the endoplasmic reticulum proteostasis network restores proteostasis of pathogenic GABAA receptors: Y.L. Fu, et al.; PLoS One 13, e0207948 (2018), Abstract
A transient rise in free Mg2+ ions released from ATP-Mg hydrolysis contributes to mitotic chromosome condensation: K. Maeshima, et al.; Curr. Biol. 28, 444 (2018), Abstract
Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale.: Morgenstern, M., Stiller, S. B., et al.; Cell Rep. 19, 2836 (2017), Abstract
Dynamic organization of chromatin domains revealed by super-resolution live-cell imaging: T. Nozaki, et al.; Mol. Cell 67, 282 (2017), Abstract
SIRT1 controls cell proliferation by regulating contact inhibition: E.H. Cho, et al.; Biochem. Biophys. Res. Commun. 478, 868 (2016), Application(s): Transfection and establishment of stable SIRT1-overexpression cell line, Abstract
Positive regulation of the enzymatic activity of gastric H+,K+-ATPase by sialylation of its β-subunit: T. Fujii, et al.; Biochim. Biophys. Acta 1858, 1228 (2016), Application(s): Cell culture, Abstract
The Detection of Neomycin Phosphotransferase Activity in Plant Extracts
from the bacterial transposon Tn5, the gene product (E.C.2.7.1.95) transfers the γ phosphate group of ATP to a specific hydroxyl group of aminoglycosidic antibiotics. In this way, it detoxifies neomycin, kanamycin, G418, as well as paromomycin. Plants expressing
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
. Moreover, the Xgal product and the X-P/NBT precipitate produced by PLAP often are too dark to allow simultaneous detection of another colored precipitate produced by immunohistochemical detection of a cellular antigen. However, occasionally, this will work (e.g
Invitrogen的王牌：Lipofectamine 2000转染试剂
药物抗性的筛选标记基因一起转染目的基因是建立稳定转染细胞系最常用的方法。氨基糖苷磷酸转移酶基因(APH或neor)可以合成APH酶，通过磷酸化使药物失活，从而提供对GENETCIN选择性抗生素(G418 Sulfate)的抗性。抗生素抗性基因可以与目的基因在同一个质粒上，也可以在不同的质粒上。如果两个不同的质粒同时转染，两个质粒都可能整合形成稳定转化子。对于两种不同质粒的共转染，带有目的基因的质粒和带有筛选标记的质粒间的比例为3:1或更高以保证抗性克隆带有转染的目的基因。瞬时转染效率的改进一般也会
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