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文献和实验Preparation and Ligand Screening of a gt11 Lysogen Library
protein, a host strain is used that carries a deletion in the gene for the Ion protease. λgtl 1 can also form plaques if grown on a host that carries an amber suppressor mutation (for a recent review on the λgtll system, see ref. 3 )
Off-Target Networks Derived from Ligand Set Similarity
Chemically similar drugs often bind biologically diverse protein targets, and proteins with similar sequences or structures do not always recognize the same ligands. How can we uncover the pharmacological relationships among proteins
Quantitative Determination of Protein Stability and Ligand Binding by Pulse Proteolysis
also can be used to determine the affinity of a ligand to its protein target by monitoring the change in protein stability upon ligand binding. The Basic Protocol describes the detailed procedure for determining protein stability using pulse proteolysis. For pulse
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