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- 技术资料
- 英文名:
20S Proteasome (human) (untagged)
- 保质期:
见包装说明
- 保存条件:
-80°C
- 库存:
99
- 供应商:
欣博盛生物
- 规格:
50 ug
20S Proteasome (human) (untagged)产品由Adipogen公司生产,产地是瑞士。该产品货号为SBB-PP0005-C050,包装规格是50 ug。如需购买该产品,询问折扣价/货期/技术问题,请联系Adipogen授权代理商——欣博盛生物
More Information
Product Details
Product Type Protein
Properties
Source/Host Human Erythrocytes
Sequence
Human 20S Proteasome.
Crossreactivity Human
Application
Able to proteolytically degrade substrates in an ATP-independent manner. The 20S core can be activated chemically with SDS (0.035%) or by the addition of PA28. Initial experiments should be carried out at 20S proteasome concentrations between 1-5nM.
MW ~700kDa
Purity ≥95% (SDS-PAGE)
Concentration Lot dependent.
Formulation Liquid. In 50mM HEPES pH 7.6, 100mM sodium chloride, 1mM DTT.
Other Product Data
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -80°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
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文献和实验请咨询Adipogen授权代理商欣博盛生物,或访问Adipogen官方网站查看。
each of seven different α and β subunits arranged into four stacked rings (α7 β7 β7 α7 ). Separation by two-dimensional (2D) gel electrophoresis of the human erythrocytes 20S proteasome subunits and mass spectrometry (MS) identification of all the observed spots reveal
Purification and Proteomic Analysis of 20S Proteasomes from Human Cells
of proteomic approaches to study the subunit composition of 20S proteasome complexes purified from human cells. An immunoaffinity purification method is presented. The separation of all 20S proteasome subunits by 2D gel electrophoresis and the subunit
Assays for Proteasome Inhibition
activity of the proteasome is contained within this central 20S core (MW=730 kDa), a multicatalytic protease that has three well characterized peptidase activities. The three peptidases: chymotryptic, tryptic, and postglutamyl peptide hydrolytic activities
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