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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Progranulin (human) (rec.) (untagged)
- 保质期:
见包装说明
- 保存条件:
4°C
- 库存:
99
- 供应商:
欣博盛生物
- 规格:
50 ug/10 ug
| 规格: | 50 ug | 产品价格: | ¥5640.0 |
|---|---|---|---|
| 规格: | 10 ug | 产品价格: | ¥2400.0 |
Progranulin (human) (rec.) (untagged)产品由Adipogen公司生产,产地是瑞士。该产品货号为AG-40A-0188Y-C050,包装规格是50 ug。如需购买该产品,询问折扣价/货期/技术问题,请联系Adipogen授权代理商——欣博盛生物
Synonyms Proepithelin; PEPI; PC Cell-derived Growth Factor
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence
Signal peptide and human progranulin (aa 1-593) is untagged. Reflects the native sequence with no additional aa.
Crossreactivity Human
Biological Activity
Binds to human sortilin. Activates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight (see reference 1).
MW ~90kDa (glycosylated protein by SDS-PAGE)
Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 50µg size: 1 mg/ml
Reconstitution 10µg size: Reconstitute with 100µl sterile endotoxin-free water.
50µg size: Reconstitute with 50µl sterile endotoxin-free water.
Formulation Lyophilized. Contains PBS.
Other Product Data
Uniprot link P28799: Granulins (human) [Precursor]
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
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文献和实验- Involvement of Progranulin in Hypothalamic Glucose Sensing and Feeding Regulation: H.K. Kim, et al.; Endocrinology 152, 4672 (2011)
- Progranulin directly binds to the CRD2 and CRD3 of TNFR extracellular domains: J. Jian, et al.; FEBS Lett. 587, 3428 (2013)
- Progranulin and a five transmembrane domain-containing receptor-like gene are the key components in receptor activator of nuclear factor B (RANK)-dependent formation of multinucleated osteoclasts: J. Oh, et al.; J. Biol. Chem. 290, 2042 (2015)
- New discovery rarely runs smooth: an update on progranulin/TNFR interactions: B.C. Wang, et al.; Protein Cell 6, 792 (2015)
- Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes: Y. Tanaka, et al.; Hum. Mol. Genet. 26, 969 (2017)
- SorCS2 binds progranulin and regulates motor axon outgrowth: PB. Thomasen, et al.; Cell Rep. 42, 113333 (2023)
, especially in the field of cancer treatment. In this chapter, a receptor localization technique is described which utilises frozen sections of human tissue and combines immunohistochemistry (IHC) and micro-autoradiography. IHC utilises antibodies tagged
Purification, Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase
We have compared the purification procedures as well as the biochemical and kinetic properties of wild type (wt-SAL3), untagged recombinant (rec(−His)SAL3), and tagged recombinant (rec(+His)SAL3) purified forms of Staphylococcus aureus lipase
, A., Férec, C., Vasquez, K.M., Kehrer‐Sawatzki, H., and Chen, J.M. 2011. On the sequence‐directed nature of human gene mutation: The role of genomic architecture and the local DNA sequence environment in mediating gene mutations underlying human inherited
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