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文献和实验into the wells of the SDS-PAGE gel. Load an appropriate amount of Trident Blue Prestained Protein Ladder or Trident Prestained Protein Ladder to one or more additional lanes.2. Run the gel in 1X Trident Running Buffer for 1-2 hours at 50-100 V.We recommend
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
that bridge distant genomic regions, while simultaneously identifying such physical proximities. This method is also useful for determining if a candidate protein might mediate long-range interactions, both in cis and in trans in the nucleus. We discuss
Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles
. If the protein has not been purified previously, the first step is to solubilize the membrane preparation with a range of detergents and to perform assays of function on the solubilized material (see also Chapter 22 of Biomembrane Protocols: I. Isolation
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