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- Cell Signaling Technology
- USA
- 59329P
- 2025年07月14日
- W
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文献和实验Coomasie Blue Staining of Protein Gels
LEVEL I Materials Protein gel from Exercise 4.2 0.25% (w/v) Coomasie Brilliant Blue R 250 in methanol-water-glacial acetic acid (5-5-1), filtered immediately before use. 7% (v/v) acetic acid Commercial
?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder
Quantitation of Protein: Coomassie Bright Blue Dye - binding Method (Bradford Method)
spectrophotometer, Test tube, Pipet, Flask 2. Reagents (1) Standard protein solution: Weigh 10mg bovine serum albumin, dissolved in distilled water then dilute to 100ml to get the 100μg/ml solution. (2) Coomassie bright blue G-250 solution
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