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文献和实验) 1-8μl Calf Thymus DNA (0.5ug/ul) 2μl TE buffer 0-7μl Total Volume 10μl Add 1ul of 4% Formic Acid and incubate for 25 min at 37℃. During this incubation, add 15 μl of stock piperidine to 135 μl
A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1. 10X Binding Buffer
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