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- LGC(Lucigen)
- 60300-1
- 2026年02月23日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
北京柏瑞图科技有限公司
- 规格：
12 rxns
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文献和实验Protein Expression and Purification Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These cells must be competent. (Protocol for how to make competent cells.) a) Take competent cell stock aliquot (about 100 m L) out of -80o C freezer. Place on ice
Mesoplasma florum: Tn5 Transposase
16820464 . The protocol also builds on the NEB IMPACT-CN protein purification kit. Materials pWH1891 plasmid (kind gift of Prof. Wolfgang Hillen) BL21(DE3)pLysS cells TEGX buffer 10 mM Tris-HCl pH 7.5 700 mM
[mrr-hsdRMS-mcrBC] ø80 lacZ Δ M15 Δ lacX74deoR recA1 araD139 Δ[ara-leu] 7697 galV galK rpsL [StrR] endA1nupG) or equivalent electrocompetent E. coli cells •
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