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- 库存:
10
- 供应商:
北京柏瑞图科技有限公司
- 规格:
4 x 100 µg
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
) (chilled) KSR medium for hESC Matrigel stock solution MEF feeder cells (irradiated CF1) as prepared in Preparation of Mouse Fibroblast Feeder Cells for Human Embryonic Stem Cell Culture (McElroy and Reijo Pera 2008a ) MEF medium
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
Recombinant human FGF basic (R&D Systems, Catalog # 233-FB , 4114-TC , or equivalent) Accutase (Innovative Cell Technologies, Catalog # AT104 or equivalent) Cultrex® Basement Membrane Extract (BME) (R&D Systems, Catalog # 3433-005-01 or equivalent
Purification of Human Multiprotein Complexes using OneSTrEP Technology
Introduction Here we describe a strategy for isolation of multiprotein complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed
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