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- BVD-S1111
- 2026年02月22日
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- 供应商：
北京柏瑞图科技有限公司
- 规格：
BVD-S1111-110assays(1kit)
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文献和实验they may be split 1:4 into fresh media. (Fungizone can be omitted from this point onwards). 2. Stage I - Preparation of collagen I from rat tails Note: Protocol for approximately 12-14 adolescent (fresh or frozen) rat tails 1) Prepare rat
Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue
weight tissue), and sub-types from kidney tissues is therefore an important part of determining the extent of renal fibrosis in ageing and diseased states. This chapter describes a colorimetric-based hydroxyproline assay used to estimate total collagen
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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