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- 文献和实验
- 技术资料
- 供应商:
北京柏瑞图科技有限公司
- 规格:
RDB-RDSM017-100Assays-100Assays
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文献和实验An Enzymatic Microplate Assay for Testing P-Glycoprotein Substrates and Inhibitors
it possible to detect specific interactions between drugs and P-gp. They took into account the existence of multiple binding sites on P-gp to finalize an optimized strategy that involves the assay of the P-gp ATPase stimulated by three reference substrates
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
anchor. First, control studies are detailed to test for nonspecific liposome immobilization (fluorescence assay; see Subheading 3.2), and to ensure that liposomes remain intact on the microplate surface (dye leakage assay; see Subheading 3.3). Finally
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