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- 2026年02月22日
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北京柏瑞图科技有限公司
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RKL-S000-55-500ug
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文献和实验peroxidase-conjugated secondary antibody for 1 hr at optimal titer (e.g., 1:20,000 in Wash Solution II) while rocking at 4°C. Wash 3 x 10 min in Wash Solution II. TIP: Alternatively, the secondary antibody
免疫组化技术-Immunohistochemical Protocols
(mostly these are IgG's), and then an appropriate pre-conjugated anti-IgG molecule to detect the primary antibody. In addition to these conventional immunostainings, a newer method has been introduced with increased sensitivity. This is called the tyramide signal
Sequential RNA and DNA fluorescence in situ hybridization
streptavidin diluted 1:100 in TNT buffer containing 3% BSA for 1 hour at RT (note 6); Wash with PBS 3 times; Incubate with fluorophore-conjugated tyramide diluted in 0.0015% H2 O2 at 1:100 for 10 minutes at RT (note 7); Wash with PBS 3 times
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