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文献和实验Comparative Protein Structure Modeling Using MODELLER
protein with its actual structure (left). (B ) A putative proteoglycan binding patch was identified on a medium‐accuracy comparative model of mouse mast cell protease 7 (right), modeled based on its 39% sequence identity to the crystallographic structure
. 1. Introduction Glycogen synthase kinase (GSK)-3 is a serine/threonine proteinkinase which was first described as a component of insulin-mediatedsignaling pathway (1) . There are two homologous mammalianisoforms, encoded by different genes, GSK-3a and GSK
on serine, threonine, and tyrosine (179). In this method, a protein or mixture of proteins is digested to peptides with a protease and then subjected to a multistep procedure for the conversion of phosphoamino acids into free sulfhydryl groups. To capture
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