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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 英文名：
Soil Extract Glucose Yeast Extract Medium
- 规格：
250mLX4
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文献和实验Yeast Extract for Friis Medium
1.Yeast (93.75 g) + Super Q Water (562.5 ml) per flask.2.Place in incubator at 37°C for 20 minutes.3.Heat to 90-100°C for 5 minutes.4.Cool on ice.5.Pour into centrifuge bottles and centrifuge at 2500 rpms for 30 minutes (4°C).6.Filter supernatant
Yeast Nuclear Extract (Small Scale)
Yeast Nuclear Extract (Small Scale) Hahn Lab (adapted from J.Leatherwood,Ptashne lab) last modified Mon,Aug 27,2001 Cell Growth 3 liters of cells are grown in YPD (3% glucose)to A600 of 3-5.Antifoam A (two drops from a P200 pipetman)is added
Invitro transcription with yeast nuclear extract
In vitro transcription with yeast nuclear extract Steve Hahn Wear gloves
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