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- 天净沙
- 苏州
- 6-0472
- 2026年02月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4-25℃
- 保质期：
1 Year
- 英文名：
Calcium Ionophore Solution
- 规格：
250mL
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文献和实验Simplified Calcium Phosphate Coprecipitation
add 61 µL of 2M CaCl2 , plus 4 - 10 µg (1 - 2.5 µL) DNA, and q.s. to 0.5 mL by adding (438 µL) H2 O (or try TE pH8.0 ). Mix with a pipet and transfer dropwise to the 2x HEPES solution. Mix and then immediately sprinkle on top of the cultured cells
Competent cells, calcium chloride method, E. coli, description
, CaCl2 method, E. coli, long protocol Keywords: competent cells; E. coli; calcium chloride; Zappe CaCl2 competent cells Description Short protocol Related Contents Contributor: : Zappe, H
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
, while gently mixing.Let sit 20min in the dark; at end, solution should look slightly cloudy.Add 1.4ml of ppt dropwise to each plate.Tilt gently to mix.Put back in 5% CO2.Incubate 5-7 hours undisturbed.GLYCEROL SHOCK:Warm 2xHeBS, PC12 Culture Medium, PBS, 50
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