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- 2026年02月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4-25℃
- 保质期：
1 Year
- 英文名：
Base Buffer (2x)
- 规格：
250mL
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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
CCMB buffer to make chemical competent cell
but that amount was found to be 2X too much per tests on 1.23.07 --Meaganl 15:50, 25 January 2007 (EST) water to 1 liter autoclave or sterile filter Test pH adjustment by mixing 4 parts ligation buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5
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